300kV Transmission Electron Microscope

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Großgerät

Kurzbeschreibung

The 300 kV TEM FEI Tecnai F30 Helium "Polara" is a cryo transmission electron microscope dedicated to visualizing biological samples as close to their native state as possible. It is unique in Austria and equipped with the most advanced imaging systems, was funded by a Vienna Spot of Excellence grant and became fully operational at the beginning of 2008. Since 2015 it is equipped with a new camera (Gatan K2 summit direct electron detector) capable of up to near atomic resolution. Additionally an alternative camera system with an energy filter can be used for elemental analysis of samples by Electron Energy Loss Spectroscopy (EELS).

Ansprechperson

Thomas Heuser

Research Services

Visualisation of:
- biological samples close to their native state
- cells and tissues in 2D and 3D
- molecules, micro-organisms, organelles, small synthetic structures

Sample preparation:
- cryopreparation:
+ immersion (cryo plunge) freezing
+ HPF, FS
+ Tokuyasu
- conventional fixation
- immunolabelling
- ultramicrotomy
- correlative microscopy
- negative staining
- rotary shadowing
- chemical or critical point drying
- sputter coating

Methoden & Expertise zur Forschungsinfrastruktur

The Polara is beeing used for:
- Cryo samples
- Tomography (room temperature and cryo)
- Single Particle cryo EM
- Analytical EM (EELS)

Zuordnung zur Core Facility

Electron Microscopy

Please contact Thomas Heuser (head of the EM Facility) for questions or more information.
Thomas Heuser
Vienna Biocenter Core Facilities (VBCF)
+43 1 796 2324 7120
thomas.heuser@vbcf.ac.at
http://www.vbcf.ac.at/em
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.