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Pacific Biosciences Sequel instrument
Long read sequencing (average read length ~10kb), max. 400.000 reads
Advice and guidance of sequencing projects are offered by our team, that relys on five years of experience with sequencing systems and sequencing data analysis. All common sequencing applications (ChIP-seq, RNA-seq, Whole Genome Sequencing etc.) are supported and the development of novel methods and protocols encouraged.
Depending on the required application, library preparation can either be performed by the users or by the facility. Multiplexed, barcoded samples can be used to fully exploit the sequencing capacity of the systems. We provide sample preparation protocols and offer contact to Illumina or reference customers on demand. To ensure optimal sequencing results we have introduced quality control steps at several points of the sequencing procedure.
Base calling and primary analysis is standardly applied to the sequence reads resulting in bam/fastq files and quality information.
Our Core Facility team set up a selection of bioinformatics tools in order to assist you in further data analysis. Additionally, to these programs we run a Galaxy instance as an free to use service for our users.
Methoden & Expertise zur Forschungsinfrastruktur
DNA and RNA pipelines for:
- Sequencing the genome of a species
- Cataloging variation between individuals in a species
- Characterizing differences between cells within an individual
- Describing the underlying cellular mechanisms (mRNA expression levels, defining binding sites of DNA-binding molecules and many other)