Universität Salzburg
Salzburg | Website
Kurzbeschreibung
Zusammenfassung diverser Geräte und Anlagen der Core Facility Bioanalytik: diverse chromatographische und elektrovorretische Trennapparaturen mit verschiedenen Detektionssystemen (UV-Vis Spektroskopie, Fluoreszenz, Lichtstreuung), Gaschromatographie-Massenspektrometrie, Messgeräte für Proteineigeschaften (Zirular-Dichrosimus, Infrarot Absorption), UV-Spektrometer, Massenspektrometer (Elektrospray Ionisation, Quadrupol-Orbitrap Massenanalyse), Einrichtungen für Probenvorbereitung und -Aufbewahrung (Kühl- und Gefriereinheiten, Inkubatoren, Schüttler, Zentrifugen)
Ansprechperson
Prof. Dr. Christian Huber
Research Services
Proteom- und Metabolommessungen; Charakterisireung von Proteinen und Protein-Modifikationen; Strukturanalyse von kleinen Molekülen; quantitative Analyse von ausgewählten Biomolekülen in biologischen Matrices, biologische Netzwerkanalyse, Prozessanalyse
Methoden & Expertise zur Forschungsinfrastruktur
In dieser Core Facility sind jene Geräte und Anlagen erfasst, die als Infrastruktur für den laufenden Betrieb sowie für den Betrieb der Großgeräte in der Proteomik Facility notwendig sind. Dies umfasst Zellherstellung und Zellkultur, Zellaufarbeitung, Aufarbeitung biologischer Proben, die Charakterisierung von Zellen, zellulären Proteomen und Metabolomen, sowie die gezielte Analyse ausgewählter Biomoleküle. Des Weiteren umfasst es geeignete Vorrichtungen zur sicheren Lagerung und Aufbewahrung von Zellen und biologischen Proben.
Equipment der Core Facility
Bitte kontaktieren Sie uns unter science.plus@sbg.ac.at, oder kontaktieren Sie direkt die/den FI-Verantwortliche/n.
Technische Universität Wien, Institute of Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering und Institute of Chemical Technologies and Analytics, Research Group Bioanalytics;
Universität Tübingen, Applied Bioinformatics Group;
Freie Universität Berlin, Algorithmische Bioinformatik;
Paracelsus Medizinische Privatuniversität und Universitätsklinikum, Salzburg, Universitätsklinik für innere Medizin III und Institut für Experimentelle und Klinische Zelltherapie;
Medizinische Universität Innsbruck, Sektion für Physiologie;
Medizinische Universität Wien, Abteilung Immunpathologie;
University of Amsterdam, Academic Medical Center;
Federal University of Rio de Janeiro
Universität Tübingen, Applied Bioinformatics Group;
Freie Universität Berlin, Algorithmische Bioinformatik;
Paracelsus Medizinische Privatuniversität und Universitätsklinikum, Salzburg, Universitätsklinik für innere Medizin III und Institut für Experimentelle und Klinische Zelltherapie;
Medizinische Universität Innsbruck, Sektion für Physiologie;
Medizinische Universität Wien, Abteilung Immunpathologie;
University of Amsterdam, Academic Medical Center;
Federal University of Rio de Janeiro
MARINA: Managing the risks of Nanoparticles
2008-2013
Lang Tran, Koordinator
Commission of the European Communities
http://www.marina-fp7.eu/
Doktoratskolleg Immunity in Cancer and Allery; W 1213
2008-2017
Christian Huber, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
http://ica.sbg.ac.at/
Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
2013-2020
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
http://cdl-biosimilars.sbg.ac.at/
DigiTheraeutX: Digitalized Production of Biotherapeutics
2022-2027
C. Huber/C. Herwig/R. Birner-Grünberger
FWF
https://www.plus.ac.at/biowissenschaften/forschung/digitherapeutx
2008-2013
Lang Tran, Koordinator
Commission of the European Communities
http://www.marina-fp7.eu/
Doktoratskolleg Immunity in Cancer and Allery; W 1213
2008-2017
Christian Huber, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
http://ica.sbg.ac.at/
Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
2013-2020
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
http://cdl-biosimilars.sbg.ac.at/
DigiTheraeutX: Digitalized Production of Biotherapeutics
2022-2027
C. Huber/C. Herwig/R. Birner-Grünberger
FWF
https://www.plus.ac.at/biowissenschaften/forschung/digitherapeutx
Proteomics and immunoblotting analyses reveal antigens that optimize the immunodiagnosis of the infection by Toxocara spp.
2022
Márcia Barbosa da Silva, Antônio Márcio Santana Fernandes, Eduardo Santos da Silva, Juan Ricardo Urrego, Leonardo Freire Santiago, Luís Fabián Salazar Garcés, Ricardo Dias Portela, Luis GC Pacheco, Peter Briza, Fátima Ferreira, Carina Silva Pinheiro, Neuza Maria Alcantara‐Neves
Transboundary and Emerging Diseases 7
DOI:10.1111/tbed.14650
At-line quantitative profiling of monoclonal antibody products during bioprocessing using HPLC-MS
2022
Böttinger, K., Esser-Skala, W., Herwig, C., Huber, C. G.
Analytica Chimica Acta (2022) 1207: 339813
https://doi.org/10.1016/j.aca.2022.339813
Proteolytic Profiling of Streptococcal Pyrogenic Exotoxin B (SpeB) by Complementary HPLC-MS Approaches
2021
Blöchl, C., Holzner, C., Luciano, M., Bauer, R., Horejs-Höck, J., Eckard, U., Brandstetter, H., Huber, C. G.
International Journal of Molecular Sciences 23 (2021) 412
https://doi.org/10.3390/ijms23010412
Structural Alterations of Antigens at the Material Interface: An Early Decision Toolbox Facilitating Safe-by-Design Nanovaccine Development
2021
Litty Johnson, Lorenz Aglas, Wai Tuck Soh, Mark Geppert, Sabine Hofer, Norbert Hofstätter, Peter Briza, Fatima Ferreira, Richard Weiss, Hans Brandstetter, Albert Duschl, Martin Himly
Int. J. Mol. Sci. 2021, 22(19), 10895
https://doi.org/10.3390/ijms221910895
Multiomics assessment in Enchytraeus crypticus exposed to Ag nanomaterials (Ag NM300K) and ions (Ag NO3) - Metabolomics, proteomics (& transcriptomics)
2021
Maria, V. L., Licha, D., Scott-Fordsmand, J. J., Huber, C. G., Amorim, M. J. B
Environmental Pollution 286 (2021) 117571
https://doi.org/10.1016/j.envpol.2021.117571
Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin Employing Mass Spectrometry
2021
Lebede, M., Di Marco, F., Esser-Skala, W., Hennig, R., Wohlschlager, T., Huber, C. G.
Analytical Chemistry 93 (2021) 10424–10434,
https://doi.org/10.1021/acs.analchem.1c02199
Hydrogen/deuterium exchange memory NMR reveals structural epitopes involved in IgE cross-reactivity of allergenic lipid transfer proteins
2021
Zennaro, D., Wopfner, N., Huber, C. G., van Ree, R., Mari, A., Lackner, P., Ferreira, F., Schubert, M. & Gadermaier, G.
Journal of Biological Chemistry, 295(51):17398-17410
doi: 10.1074/jbc.RA120.014243
Biochemical and functional characterization of a new recombinant phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum
2020
Sarah Natalie Cirilo Gimenes, Lorenz Aglas, Sabrina Wildner, Sara Huber, Ana Carolina Portella Silveira, Daiana Silva Lopes, Renata Santos Rodrigues, Luiz Ricardo Goulart, Peter Briza, Fatima Ferreira, Veridiana de Melo Rodrigues Ávila, Gabriele Gadermaier
International Journal of Biological Macromolecules, 164 (2020) 1545-1553
https://doi.org/10.1016/j.ijbiomac.2020.07.221
Proteins and Molecular Pathways Relevant for the Malignant Properties of Tumor-Initiating Pancreatic Cancer Cells
2020
Samonig, L., Loipetzberger, A., Blöchl, C., Rurik, M., Kohlbacher, O., Aberger, F., Huber, C. G.
Cell Communication and Signaling 18 (2020), article # 99
https://doi.org/10.1186/s12964-020-00591-0
Dilute-and-Shoot Analysis of Therapeutic Monoclonal Antibody Variants in Fermentation Broth: A Method Capability Study
2019
Regl, C., Wohlschlager, T., Esser-Skala, W., Wagner, I., Samonig, M., Holzmann, J., Huber, C. G
mAbs 11:3 (2019) 569-582
DOI: 10.1038/s41598-018-37624-1
Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals
2018
Wohlschlager, T., Scheffler, K., Forstenlehner, I. C., Skala, W., Senn, S., Damoc, E., Holzmann, J., Huber, C. G.
Nature Communications 9 (2018) 1713
DOI: 10.1038/s41467-018-04061-7
Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells
2017
Strasser, L., Dang, H.-H., Schwarz, H., Ferreira, F., Horejs-Höck, J., Huber, C. G.
Scientific Reports 7 (2017) 16638
DOI : 10.1038/s41598-017-16726-2
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
2013
Wilmes, A., Limonciel, A., Aschauer, L., Moenks, K., Bielow, C., Leonard, M. O., Hamon, J., Carpi, D., Ruzek, S., Handler, A., Schmal, O., Herrgen, K., Bellwon, P., Burek, C., Truisi, G. L., Hewitt, P., Di Consiglio, E., Testai, E., Blaauboer, B. J., Guillou, C., Huber, C. G., Lukas, A., Pfaller, W., Mueller, S. O., Bois, F. Y., Dekant, W., Jennings, P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022
Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
2014
Behmüller, R., Forstenlehner, I. C., Tenhaken, R., Huber, C. G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3
Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry DOI: 10.1007/s00216-014-7746-3
2013
Neu, V., Bielow, Gostomski, I., Wintringer, R., Braun, R., Reinert, K., C., Schneider, P., Stuppner, H., Huber, C. G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j
Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
2015
Ranninger, C., Rurik, M., Limonciel, A., Ruzek, S., Reischl, R., Wilmes, A., Jennings, P., Dekant, W., Kohlbacher, O., Huber, C. G. DOI: dx.doi.org/10.1021/ac303722j
Journal of Biological Chemistry
http://www.jbc.org/cgi/doi/10.1074/jbc.M115.644146
Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
2015
Forstenlehner, I., Holzmann, J., Toll, H., Huber, C. G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029
Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
2015
Boyles, M. S. P., Kristl, T., Andosch, A., Zimmermann, M., Tran, N., Casals, E., Himly, M., Puntes, V., Huber, C. G., Lütz-Meindl, U., Duschl, A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9
2022
Márcia Barbosa da Silva, Antônio Márcio Santana Fernandes, Eduardo Santos da Silva, Juan Ricardo Urrego, Leonardo Freire Santiago, Luís Fabián Salazar Garcés, Ricardo Dias Portela, Luis GC Pacheco, Peter Briza, Fátima Ferreira, Carina Silva Pinheiro, Neuza Maria Alcantara‐Neves
Transboundary and Emerging Diseases 7
DOI:10.1111/tbed.14650
At-line quantitative profiling of monoclonal antibody products during bioprocessing using HPLC-MS
2022
Böttinger, K., Esser-Skala, W., Herwig, C., Huber, C. G.
Analytica Chimica Acta (2022) 1207: 339813
https://doi.org/10.1016/j.aca.2022.339813
Proteolytic Profiling of Streptococcal Pyrogenic Exotoxin B (SpeB) by Complementary HPLC-MS Approaches
2021
Blöchl, C., Holzner, C., Luciano, M., Bauer, R., Horejs-Höck, J., Eckard, U., Brandstetter, H., Huber, C. G.
International Journal of Molecular Sciences 23 (2021) 412
https://doi.org/10.3390/ijms23010412
Structural Alterations of Antigens at the Material Interface: An Early Decision Toolbox Facilitating Safe-by-Design Nanovaccine Development
2021
Litty Johnson, Lorenz Aglas, Wai Tuck Soh, Mark Geppert, Sabine Hofer, Norbert Hofstätter, Peter Briza, Fatima Ferreira, Richard Weiss, Hans Brandstetter, Albert Duschl, Martin Himly
Int. J. Mol. Sci. 2021, 22(19), 10895
https://doi.org/10.3390/ijms221910895
Multiomics assessment in Enchytraeus crypticus exposed to Ag nanomaterials (Ag NM300K) and ions (Ag NO3) - Metabolomics, proteomics (& transcriptomics)
2021
Maria, V. L., Licha, D., Scott-Fordsmand, J. J., Huber, C. G., Amorim, M. J. B
Environmental Pollution 286 (2021) 117571
https://doi.org/10.1016/j.envpol.2021.117571
Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition along Different Structural Levels of Human Chorionic Gonadotropin Employing Mass Spectrometry
2021
Lebede, M., Di Marco, F., Esser-Skala, W., Hennig, R., Wohlschlager, T., Huber, C. G.
Analytical Chemistry 93 (2021) 10424–10434,
https://doi.org/10.1021/acs.analchem.1c02199
Hydrogen/deuterium exchange memory NMR reveals structural epitopes involved in IgE cross-reactivity of allergenic lipid transfer proteins
2021
Zennaro, D., Wopfner, N., Huber, C. G., van Ree, R., Mari, A., Lackner, P., Ferreira, F., Schubert, M. & Gadermaier, G.
Journal of Biological Chemistry, 295(51):17398-17410
doi: 10.1074/jbc.RA120.014243
Biochemical and functional characterization of a new recombinant phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum
2020
Sarah Natalie Cirilo Gimenes, Lorenz Aglas, Sabrina Wildner, Sara Huber, Ana Carolina Portella Silveira, Daiana Silva Lopes, Renata Santos Rodrigues, Luiz Ricardo Goulart, Peter Briza, Fatima Ferreira, Veridiana de Melo Rodrigues Ávila, Gabriele Gadermaier
International Journal of Biological Macromolecules, 164 (2020) 1545-1553
https://doi.org/10.1016/j.ijbiomac.2020.07.221
Proteins and Molecular Pathways Relevant for the Malignant Properties of Tumor-Initiating Pancreatic Cancer Cells
2020
Samonig, L., Loipetzberger, A., Blöchl, C., Rurik, M., Kohlbacher, O., Aberger, F., Huber, C. G.
Cell Communication and Signaling 18 (2020), article # 99
https://doi.org/10.1186/s12964-020-00591-0
Dilute-and-Shoot Analysis of Therapeutic Monoclonal Antibody Variants in Fermentation Broth: A Method Capability Study
2019
Regl, C., Wohlschlager, T., Esser-Skala, W., Wagner, I., Samonig, M., Holzmann, J., Huber, C. G
mAbs 11:3 (2019) 569-582
DOI: 10.1038/s41598-018-37624-1
Native mass spectrometry combined with enzymatic dissection unravels glycoform heterogeneity of biopharmaceuticals
2018
Wohlschlager, T., Scheffler, K., Forstenlehner, I. C., Skala, W., Senn, S., Damoc, E., Holzmann, J., Huber, C. G.
Nature Communications 9 (2018) 1713
DOI: 10.1038/s41467-018-04061-7
Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells
2017
Strasser, L., Dang, H.-H., Schwarz, H., Ferreira, F., Horejs-Höck, J., Huber, C. G.
Scientific Reports 7 (2017) 16638
DOI : 10.1038/s41598-017-16726-2
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
2013
Wilmes, A., Limonciel, A., Aschauer, L., Moenks, K., Bielow, C., Leonard, M. O., Hamon, J., Carpi, D., Ruzek, S., Handler, A., Schmal, O., Herrgen, K., Bellwon, P., Burek, C., Truisi, G. L., Hewitt, P., Di Consiglio, E., Testai, E., Blaauboer, B. J., Guillou, C., Huber, C. G., Lukas, A., Pfaller, W., Mueller, S. O., Bois, F. Y., Dekant, W., Jennings, P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022
Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
2014
Behmüller, R., Forstenlehner, I. C., Tenhaken, R., Huber, C. G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3
Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry DOI: 10.1007/s00216-014-7746-3
2013
Neu, V., Bielow, Gostomski, I., Wintringer, R., Braun, R., Reinert, K., C., Schneider, P., Stuppner, H., Huber, C. G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j
Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
2015
Ranninger, C., Rurik, M., Limonciel, A., Ruzek, S., Reischl, R., Wilmes, A., Jennings, P., Dekant, W., Kohlbacher, O., Huber, C. G. DOI: dx.doi.org/10.1021/ac303722j
Journal of Biological Chemistry
http://www.jbc.org/cgi/doi/10.1074/jbc.M115.644146
Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
2015
Forstenlehner, I., Holzmann, J., Toll, H., Huber, C. G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029
Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
2015
Boyles, M. S. P., Kristl, T., Andosch, A., Zimmermann, M., Tran, N., Casals, E., Himly, M., Puntes, V., Huber, C. G., Lütz-Meindl, U., Duschl, A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9