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Applied Biosystems 3130xl Genetic Analyser is the latest generation of 16-capillary electrophoresis instruments and is ideal for medium throughput projects. The system offers industry-leading performance, plus sophisticated automation capabilities allowing researchers to save time, reduce costs and increase productivity. The instrument can be used to provide sequence data or microsatellite genotypes (fragment analysis) from PCR amplified DNA samples. The samples can be run on a 50cm array which gives sequence reads or fragment genotypes up to about 900+basepairs. Microsatellite samples can be multiplexed in pools of markers such that many loci can be genotyped at once using 4 different fluorescent dyes and differing fragment lengths.
Dr. Steve Smith
Methods & Expertise for Research Infrastructure
The ABI3130xl genetic analyser is ideal for standard DNA sequencing and microsatellite fragment analysis. The lab routinely conducts estimates of mating systems and parentage in natural populations. We also investigate the phylogenetic relationships between populations and species from many parts of the world. Our genetic expertise covers a wide spectrum of taxa including birds, mammals, fish, insects and bacteria. We are experienced in wildlife forensic applications and routinely work with problematic tissues such as dried blood, faeces, feathers, buccal swabs and hair samples.