BioIndustrial Pilot Plant

University of Natural Resources and Life Sciences Vienna (BOKU)

Wien | Website

Core facility (CF)

Short Description

The BioIndustrial Pilotplant at BOKU was engineered according to GMP guidelines and comprises a modular fermentation plant for cultivation of mamalian and microbial cells on industrial scale.

For purification of products a disc stack separator, centrifuges, high pressure homogenizer, depth-, ultra- and dia-filtration units, and chromatographic systems are available.

Contact Person

DI Dr. Markus Luchner

Research Services

* Process development for up- and downstream processing with different host organism (bacterial, yeast, cell
culture,…)
* Fermentation scale up from µ- to lab- and pilot scale
* On-line monitoring platform and development of monitoring strategies for critical control points in real-time in
up- and downstream
* Manufacturing and processing of products at pilot scale (non-GMP, but under “GMP-like” conditions)
* Available in-house model systems (e.g. rhSOD, GFP, scFv (IB or soluble)) and model organisms (e.g. E. coli,
P. pastoris, CHO, Sf9)
* Open for model systems and organisms provided by partners
* Method development for cell harvest, IB harvest, refolding, precipitation; small scale development and transfer
to pilot scale
* Filter testing for Depth- and Sterile filtration including upscale
* Ultra-/Diafiltration, method development and upscale; membrane testing
* Chromatographic separation; method development and upscale; benchmarking of different resins with in-house
model systems (different feed)
* Test of equipment provided by supplier

Methods & Expertise for Research Infrastructure

* Process development for up- and downstream processing with different host organism (bacterial, yeast, cell culture,…)
* Fermentation scale up from µ- to lab- and pilot scale
* On-line monitoring platform and development of monitoring strategies for critical control points in real-time in up- and downstream
* Manufacturing and processing of products at pilot scale (non-GMP, but under “GMP-like” conditions)
* Available in-house model systems (e.g. rhSOD, GFP, scFv (IB or soluble)) and model organisms (e.g. E. coli, P. pastoris, CHO, Sf9)
* Open for model systems and organisms provided by partners
* Method development for cell harvest, IB harvest, refolding, precipitation; small scale development and transfer to pilot scale
* Filter testing for Depth- and Sterile filtration including upscale
* Ultra-/Diafiltration, method development and upscale; membrane testing
* Chromatographic separation; method development and upscale; benchmarking of different resins with in-house model systems (different feed)
* Test of equipment provided by supplier

Dr. Markus Luchner leitet die Bioindustrielle Pilotanlage am Standort des VIBT / DBT. Sein Hintergrund ist die Bioverfahrenstechnik und Biotechnologie.
DI Dr. Markus Luchner
Department of Biotechnology / BioIndustrial Pilot Plant
+43 1 47654 6672
Markus.Luchner@boku.ac.at
http://www.biotec.boku.ac.at/arbeitsgruppenresearch-groups/bio-industrial-pilotplant/
https://portal.meril.eu/meril/view/facilitys/15478
The Pilot Plant is open for research projects and research-driven educational programs.
For further information please contact Dr. Markus Luchner (markus.luchner@boku.ac.at).
TU Vienna
Univeristy of Applied Sciences Vienna
Industrial companies