DNA Sequencer II

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Large equipment

Short Description

Illumina HiSeq 2500
http://www.illumina.com/systems/hiseq_2500_1500.html

Maximal read length is 2x150bp yielding an maximum of 75Gbp quality filtered reads per lane.

Contact Person

Andreas Sommer

Research Services

Advice and guidance of sequencing projects are offered by our team, that relys on five years of experience with sequencing systems and sequencing data analysis. All common sequencing applications (ChIP-seq, RNA-seq, Whole Genome Sequencing etc.) are supported and the development of novel methods and protocols encouraged.

Depending on the required application, library preparation can either be performed by the users or by the facility. Multiplexed, barcoded samples can be used to fully exploit the sequencing capacity of the systems. We provide sample preparation protocols and offer contact to Illumina or reference customers on demand. To ensure optimal sequencing results we have introduced quality control steps at several points of the sequencing procedure.

Base calling and primary analysis is standardly applied to the sequence reads resulting in bam/fastq files and quality information.

Our Core Facility team set up a selection of bioinformatics tools in order to assist you in further data analysis. Additionally, we run a Galaxy instance as an free to use service for our users.

Methods & Expertise for Research Infrastructure

- Sequencing the genome of a species
- Cataloging variation between individuals in a species
- Characterizing differences between cells within an individual
- Describing the underlying cellular mechanisms (mRNA expression levels, defining binding sites of DNA-binding molecules and many other)

Allocation to Core Facility

Next Generation Sequencing

Andreas Sommer
Vienna Biocenter Core Facilities (VBCF)
+43 1 7962324 7030
andreas.sommer@vbcf.ac.at
http://www.vbcf.ac.at/ngs
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.