University of Salzburg

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Short Description

The triple-quadrupole technology is currently the most robust and accurate technique for quantitatively analyzing analytes in very complex matrices. The device is therefore used for the elaboration of quantification methods of biomarkers in biological samples, e.g. serum, plasma or cell extracts and is an important prerequisite for cooperations with the pharmaceutical industry. Subsequently, the methods will be used for the validation of toxicity markers (two major EU projects).

Contact Person

Prof. Dr. Christian Huber

Research Services

HPLC-MS measurements

Methods & Expertise for Research Infrastructure

Quantification of biomolecules
Validation of target molecules in biological samples
Highly sensitive detection of proteins in cell extracts

Allocation to Core Facility


Prof. Dr. Christian Huber
Fachbereich Biowissenschaften, Schwerpunkt ACBN
0043 662 8044 5738
Please contact us via science.plus@sbg.ac.at, or contact the responsible person for this section, mentioned in the contact field
Ever Neuro Pharma, Unterach, Österreich
Predict-IV – Profiling the toxicity of new drugs; a non animal-based approach integrating toxicodynamics and biokinetics
Wolfgang Dekant, Koordinator
Commission of the European Communities

MARINA: Managing the risks of Nanoparticles
Lang Tran, Koordinator
Commission of the European Communities

Doktoratskolleg Immunity in Cancer and Allery; W 1213
Josef Thalhammer, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung

Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
Wilmes A., Limonciel A., Aschauer L., Moenks K., Bielow C., Leonard M.O., Hamon J., Carpi D., Ruzek S., Handler A., Schmal O., Herrgen K., Bellwon P., Burek C., Truisi G.L., Hewitt P., Di Consiglio E., Testai E., Blaauboer B.J., Guillou C., Huber C.G., Lukas A., Pfaller W., Mueller S.O., Bois F.Y., Dekant W., Jennings P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022

Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
Behmüller R., Forstenlehner I.C., Tenhaken R., Huber C.G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3

Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry
Neu V., Bielow C., Gostomski I., Wintringer R., Braun R., Reinert K., Schneider P., Stuppner H., Huber C.G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j

Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
Ranninger C., Rurik M., Limonciel A., Ruzek S., Reischl R., Wilmes A., Jennings P., Dekant W., Kohlbacher O., Huber C.G.
Journal of Biological Chemistry

Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
Forstenlehner I., Holzmann J., Toll H., Huber C.G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029

Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
Boyles M.S.P., Kristl T., Andosch A., Zimmermann M., Tran N., Casals E., Himly M., Puntes V., Huber C.G., Lütz-Meindl U., Duschl A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9