Lattice Light Sheet (LLS) Microscope

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Large equipment

Short Description

Unlike conventional microscopy, light sheet microscopy makes use of two perpendicular lenses (one for exciting a thin sheet of light in the sample) while the other images this illuminated plane. It thereby can achieve a very high contrast and significantly reduced phototoxic effects (since less of the sample is illuminated per image), all while also achieving very fast volume imaging rates. It is in many applications considered more desirable than Spinning disk confocal microscopy for studying fast processes on the microscale. The lattice light sheet microscope is a special type of light sheet fluorescence microscope, based on a design first demonstrated in the lab of Dr. Eric Betzig at HHMS Janelia Farm (MD, USA), suitable for fast 3d imaging of live cells with very high resolution and minimal phototoxicity. Its key feature is that it creates a structured (as opposed to homogeneous) light sheet which has been shown results further resolution improvements and reduction in required laser illumination intensity (lower phototoxicity). The setup is equipped with 4 laser lines (for imaging with 4 different fluorescence channels) and prime applications are in cell biology - for studying sub-cellular dynamics of fluorescently labelled molecules in live cells in a minimally invasive fashion.

Contact Person

Kareem Elsayad

Research Services

-Powerful workstation with various software suitable for data analysis (connected to microscope workstation via 10G connectionf or fast data transfer)
-Staff experienced with light sheet microscopy, and associated data analysis and representation

Methods & Expertise for Research Infrastructure

-Powerful workstation with various software suitable for data analysis (connected to microscope workstation via 10G connectionf or fast data transfer)
-Staff experienced with light sheet microscopy, and associated data analysis and representation

Allocation to Core Facility

Advanced Microscopy

Head of Advanced Microscopy
Kareem Elsayad
Vienna Biocenter Core Facilities (VBCF)
+43 1 79730 7110
kareem.elsayad@vbcf.ac.at
http://www.vbcf.ac.at/advmicro
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.
Daniel Gerlich (IMBA)
Alipasha Vaziri (IMP & Rockefeller University, NY)