Liquid chromatograph

University of Salzburg

Salzburg | Website

Large equipment

Short Description

Capillary and nano-liquid chromatograph for operation at flow rates from 0.05 to 200 μL / min, column thermostat, UV detector with 3-250 nL measuring cells, auto-injector for volumes of 1-250 μL, control and evaluation with Chromeleon 7, computer

Contact Person

Prof. Dr. Christian Huber

Research Services

HPLC measurements

Methods & Expertise for Research Infrastructure

The system is used as a sample preparation technique for protein and proteome analysis. The separated proteins or peptides are usually transferred directly into a mass spectrometer for the determination of the exact mass and for the generation and mass measurement of fragments. The methodology is used in two EU projects (Predict IV and MARINA) for the differential proteome analysis as well as in various cooperation projects for protein and proteome characterization.

Allocation to Core Facility


Prof. Dr. Christian Huber
Fachbereich Biowissenschaften, Schwerpunkt ACBN
0043 662 8044 5738
Please contact us via, or contact the responsible person for this section, mentioned in the contact field
Predict-IV – Profiling the toxicity of new drugs; a non animal-based approach integrating toxicodynamics and biokinetics
Wolfgang Dekant, Koordinator
Commission of the European Communities

MARINA: Managing the risks of Nanoparticles
Lang Tran, Koordinator
Commission of the European Communities

Doktoratskolleg Immunity in Cancer and Allery; W 1213
Josef Thalhammer, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung

Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
Wilmes A., Limonciel A., Aschauer L., Moenks K., Bielow C., Leonard M.O., Hamon J., Carpi D., Ruzek S., Handler A., Schmal O., Herrgen K., Bellwon P., Burek C., Truisi G. L., Hewitt P., Di Consiglio E., Testai E., Blaauboer B.J., Guillou C., Huber C.G., Lukas A., Pfaller W., Mueller S.O., Bois F.Y., Dekant W., Jennings P.
Journal of Proteomics

Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
Behmüller R., Forstenlehner I.C., Tenhaken R., Huber C.G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3

Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry
Neu V., Bielow C., Gostomski I., Wintringer R., Braun R., Reinert K., Schneider P., Stuppne, H., Huber C.G.
Analytical Chemistry

Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
Ranninger C., Rurik M., Limonciel A., Ruzek S., Reischl R., Wilmes A., Jennings P., Dekant W., Kohlbacher O., Huber C.G.
Journal of Biological Chemistry

Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
Forstenlehner I., Holzmann J., Toll H., Huber C.G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029

Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
Boyles M.S.P., Kristl T., Andosch A., Zimmermann M., Tran N., Casals E., Himly M., Puntes V., Huber C.G., Lütz-Meindl U., Duschl A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9