Monolith NT115 BLUE/RED

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Large equipment

Short Description

Microscale Thermophoresis (MST) is a new method developed to measure biomolecular interactions. The Monolith NT.115 Instrument uses fluorescence detection with either a blue (470/520) or red (625/680) filter. Labeling of a biomolecule with a fluorescent dye will enable measurement of the dissocation constant between the molecule and a variety of ligands (protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, etc).

Contact Person

Arthur Sedivy

Research Services

The ProTech facility will either provide training and help with the experiment design so that you can book the instrument, or we can carry out the whole experiment for you.

Methods & Expertise for Research Infrastructure

Microscale Thermophoresis is a new method to cost efficiently measure molecular interactions, especially interactions of biomolecules.

General protein - ligand interactions can be measured (protein - protein, protein - DNA/RNA, protein - small molecule/ion).

The NT.115 BLUE/RED uses fluorescence as a read-out with either the BLUE filter (exc 450 em 520) or the RED filter (exc 620 em 680) therefore one of the binding partners needs to be fluorescently labeled.

Affinities as well as binding energies and stoichiometry of the molecular interaction can be determined.

Allocation to Core Facility

Protein Technologies

Arthur Sedivy
Vienna Biocenter Core Facilities (VBCF)
+43 1 7962324 7231
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.
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