pipetting robot mosquito® LCP

University of Natural Resources and Life Sciences Vienna (BOKU)

Wien | Website

Large equipment

Short Description

mosquito® LCP is a liquid handler which makes protein crystallography screening fast and cost-effective. It brings together speed, accuracy and high precision pipetting of nanolitre volumes with zero cross-contamination from a disposable tip.

Contact Person

Georg Mlynek PhD, Elisabeth Laurent PhD

Research Services

Please contact the Core Facility via bmca@boku.ac.at

Methods & Expertise for Research Infrastructure

• flexible automation – rapid automated plate set-up for all standard crystallisation techniques: sitting drop, hanging drop, microbatch, bicelles, microseeding and additive screening
• reproducibility – down to 25 nL
• cost savings – the ability to use smaller volumes of valuable protein resulting in cost savings and enabling a larger number of screening conditions to be studied
• no configuration changes for different experiments – eliminates the need for instrument configuration changes when changing techniques or liquid viscosities
• multiple aspiration before a single dispense and microseeding, which is essential for automating additive screening
• drop precision – perfectly positioned drops for downstream imaging by placing protein and screen drops with the same head

Also, mosquito® LCP allows to dispense lipidic cubic phase (LCP) volumes as low as 25 nL, while automated calibration of syringe and pipette positioning ensures precise drop-on-drop placement to facilitate automated imaging.

• automation of membrane protein crystallisation screening, optimisation and scale-up using techniques such as LCP and bicelles

Allocation to Core Facility

Biomolecular & Cellular Analysis (BmCA)

Georg Mlynek PhD, Elisabeth Laurent PhD
Biomolecular & Cellular Analysis
+43 1 47654-35012
Please contact the Core Facility via bmca@boku.ac.at