Selective Plane Illumination Microscope (SPIM)

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Large equipment

Short Description

Selective Plane Illumination Microscope (SPIM) or Light Sheet Fluorescence Microscope (LSFM)

Contact Person

Kareem Elsayad

Research Services

The CSF Advanced Microscopy Facility provides access, training and technical support for researchers to an expanding inventory of cutting edge optical imaging techniques.

Methods & Expertise for Research Infrastructure

-Inverted geometry (light sheet incident from side, imaging from below)
-Optimized for long term plant root growth studies: Day-night lighting cycles, media can be perfused through the sample bath, temperature control
-Also suitable for cleared tissue imaging
-4 laser lines (405nm, 491nm, 514nm, 561nm)
-fast imaging (up to 100 f/s)
-Long working distance objective (8mm), long range piezo and xyz motor stage.
-occular for easy sample finding / locating regions of interest in epi-fluorescence mode
-Custom easy to use software
-stitching of images and convolution as needed also offered as service

Allocation to Core Facility

Advanced Microscopy

Kareem Elsayad
Vienna Biocenter Core Facilities (VBCF)
+43 1 79730 7110
kareem.elsayad@vbcf.ac.at
http://www.vbcf.ac.at/advmicro
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.