VIBT Bioimaging Center

University of Natural Resources and Life Sciences Vienna (BOKU)

Wien | Website

Core facility (CF)

Short Description

Are you interested in exploring the „small world”? You can visit now the bioimaging core facility of our university. The long-desired microscope center has been established in the Muthgasse complex and harbours five high-end microscopes: two video microscopes, one of them dedicated to live cell imaging; the other one is designed for total internal reflection fluorescence microscopy, two confocal laser scanning microscopes and a laser microdissection microscope.

Confocal microscopes are for better resolution in space, for example to study chromatin structure in nuclei. Video-microscopy, as its name suggests, serves as a camera to shoot movies from your samples. It provides excellent time resolution at the expense of some structural details. The live cell imaging platform is a hybrid system: one may use it like a video-microscope or, it can be transformed into a confocal one by attaching a laser scanning module to the stativ. Soon, an incubation chamber will complete the workstation to provide convenient growth temperature, CO2 and humidity for live samples. This system might become the favourite of neurologists and developmental biologists enabling them long lasting observations in natural conditions. With the arsenal of the imaging center students, professors and scientists are able to visualize, isolate and study fine structures of various objects and materials, not necessarily biological ones. It is possible to quantify and follow movements of microorganisms, organelles and even single molecules. Spatial and temporal changes of protein interactions, quantification of internal pH, redox potentials, concentration of ions, such as Calcium, and of molecules, such as glucose, will be possible to measure as long as their presence can be converted into fluorescent signals. Last but not least, one can feel the sensation of making surgery with a laser knife of the microdissecting microscope. This system allows you to separate and to collect specific regions of your sample, for example tumorous cells from tissues. By cutting a teeny-weeny part of a given specimen for further analysis, one may perform single cell DNA, RNA tests.

Contact Person

Monika Debreczeny

Research Services

Please contact the head of the Core Facility.

Methods & Expertise for Research Infrastructure

Please contact the head of the Core Facility.

Equipment

The imaging facility is open to all scientists and aims to initiate cooperations even beyond the BOKU. Welcome are cooperations to test innovative ideas and to develop new microscopic methods. Please contact the head of the Center.