Freeze Fracture System Leica EM ACE900

Institute of Science and Technology Austria (ISTA)

Klosterneuburg | Website

Großgerät

Kurzbeschreibung

Freeze fracture describes the technique of breaking a frozen specimento reveal internal structures. Freeze etching is the sublimation of surface ice under vacuum to reveal details of the fractured face that were originally hidden.

A metal / carbon mix enables the sample to be imaged in an SEM (block-face) or TEM (replica). It is used to investigate for instance cell organelles, membranes, layers and emulsions. The technique is traditionally used for biological applications, but started to develop significantly in physics and material science. Freeze fracture electron microscopy, particularly freeze replica immunolabeling (FRIL), has provided new insights into the roles of membrane proteins in dynamic cellular processes.

Ansprechperson

Ludek Lovicar

Research Services

Freeze Fracturing

Methoden & Expertise zur Forschungsinfrastruktur

Cell biology
Neuroscience

Zuordnung zur Core Facility

Electron Microscopy Facility

Manager of Electron Microscopy Facility
Ludek Lovicar
Electron Microscopy Facility
+43 2243 9000 1066
https://ist.ac.at/en/research/scientific-service-units/electron-microscopy-facility/#contact
Information on terms of use , cooperation and fees is provided upon request. All such information is defined in a scientific collaboration agreement.