Kurzbeschreibung
Das LSM 780 (780) is an inverted point laser scanning confcal microscope. It is equipped with a high sensitive array of 32 GaAsP (Gallium Arsenide) detectors (Qantum Efficiency of 45%, a significant better signal to noise ratio) and an AiryScan Detector (AiryScan, 1.7x better resolution). Since the system provides a spectral detector, it allows the free choice of detection, the separation of overlapping spectras and the possibility to do lambda stacks with subsequent unmixing of overlapping dyes.
The system supports following applications (- or combinations thereof):
• Time Series
• Multiple Positions
• Tiles
• Live Cell Imaging
• FRET- Acquisition
• FRAP- Acquisition
• Z-Stapel (3D)
• Online Intensity Measurement
• 1.7x better Resolution due to the upgraded AiryScan Detector
Technical Data:
Wide Field Illumination:
• Halogen lamp 12V, 100W
• HXP 120W
• Filter Sets: DAPI, GFP, Cy3
Laser Lines
• Laser Diode 405nm, 30mW
• Argon Laser, Multiline, 458nm, 488nm, 514nm, 25mW
• DPSS-Laser 561nm, 20mW
• HeNE-Laser 594nm, 2mW
• HeNe-Laser 633nm, 5mW
Detectors:
• PMT 1: 371-740nm
• PMT 2: 479-750nm
• Quasar (GaAsP): 416-728 in 8.3nm steps
• AiryScan :
BP 420-480 + BP495-550
BP 420-480 + BP 495-620
BP 420-480 + LP 605
BP 465-505 + LP 525
BP 495-550 + LP 570
BP 570-620 + LP 645
• Transmission Light: T-PMT
Objectives:
• 10x/ 0.3 Plan-Apochromat
• 20x/ 0.8 Plan-Apochromat
• 40x/ 1.3 Plan-Apochromat, Oil, DIC
• 40x/ 1.1 LD C-Apochromat, Water (on request)
• 40x/1.2 C-Apochromat, Water, autocorr
• 63x/ 1.4 Plan-Apochromat, Oil, DIC
• 100x/ 1.4 Plan-Apochromat, Oil, DIC
• 100x/1.46 alpha-Plan-Apochromat, Oil, DIC (on request)
Software:
• ZEN 2012 black
• Physiology Package (Analysis Software for Time Series, ROI –Analysis, Online- und Offline Calibration of Ion Concentrations)
• Multiple Time Series
• Tile and Stitching Module
• Tile and Positions Module
• Experiment Designer Modul
• FRET Module
• ROI for HDR (High Dynamic Range Acquisition
Furthermore:
• XY motorized
• Piezo Z stage
• DIC, 37°C heating chamber for Petridishes
• Chamber Slides
• 24 Well- und 6 Well Plates
• CO2 supply
• heating possibility for the 40x und 60x Objective
Fees:
28€ / h
Ansprechperson
Sabine Rauscher
Research Services
Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance
Methoden & Expertise zur Forschungsinfrastruktur
The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies.
The available systems support high resolution imaging of tissue sections or cells, 3D and 4D acquisition, visualization of protein-protein interactions (like FRET) and live cell imaging. Furthermore the facility offers workstations to the users, which allow an onsite evaluation and processing of their data with the suitable software.
In addition the area of microscopy the core facility houses a small histology unit for sample preparation. This is equipped with one cryostat and two microtomes. The microtomes are only to cut paraffin embedded material for the purpose of RNA and DNA extraction.
For performing live cell imaging the facility provides a fully equipped cell culture lab.
To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
Zuordnung zur Forschungsinfrastruktur
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
fees (LSM 780): 28 €/h