Mass Spectrometer/Thermo Fisher Scientific/OrbiTrap Eclipse Tribid

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Open for Collaboration

Kurzbeschreibung

The Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher) includes 3 mass analyzers, i.e. a quadrupole mass filter, a linear ion trap and an Orbitrap mass analyzer and 2 detectors. The instrument allows different and partially complementary fragmentation techniques, which are collision induced dissociation (CID), higher-energy collisional dissociation (HCD), electron transfer dissociation (ETD) and a combination of ETD and HCD called EThcD.
One application of this instrument is multiplex quantitation of TMT-labelled samples as this instrument offers a novel method called MultiNotch MS3 combined with real time search option, which provides a higher accuracy of TMT quantification. Additionally, we use the instrument for the analysis of peptides with labile post-translational modifications such as arginine phosphorylation.

https://www.thermofisher.com/at/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-eclipse-tribrid-mass-spectrometer.html

Ansprechperson

Karl Mechtler

Research Services

Please refer to: https://www.viennabiocenter.org/vbcf/proteomics/

Methoden & Expertise zur Forschungsinfrastruktur

Please refer to: https://www.viennabiocenter.org/vbcf/proteomics/

Zuordnung zur Forschungsinfrastruktur

Mass Spectrometry

Nutzungsbedingungen

Vienna BioCenter Core Facilities (VBCF) offer state-of-the-art research infrastructure and scientific services to academic research institutes and companies in the field of Life Sciences at Vienna BioCenter and beyond. Our general cooperation conditions are available at https://www.viennabiocenter.org/vbcf/ (Downloads – VBCF General Cooperation Conditions). Information on usage fees is provided upon request by the core facilities.