Nanoparticle Tracking Analysis Nanosight NS300 with autosampler

Austrian Centre of Industrial Biotechnology (acib)

Vienna | Website

Großgerät

Kurzbeschreibung

This machine combination is part of the “F&E Infrastrukturförderung” of the FFG. It is used for process development for production processes of bionanoparticles.
The Nanoparticle Tracking analysis NS300 (Malvern Panalytics) serves for analysis of size and concentration of particles in solutions by combining both the Brownian motion and light scattering. Each single particle is detected individually, resulting in a size distribution of hydrodynamic diameters of the complete sample.
Moreover, the Nanosight Sample Assistant (Malvern Panalytics) allows to measure samples automatically. A 96 deep-well plate can be used for running 96 measurements without personal support.

Ansprechperson

Dr. Martin Trinker

Research Services

This device is not part of a service facility but is open for scientific collaboration.
acib offers extensive and reliable partnerships with national and international research projects as well as the implementation of contract research.

Methoden & Expertise zur Forschungsinfrastruktur

The Nanoparticle Tracking Analysis setup allows to detect particles between 10 to 1000 nm in solution. The Nanosight NS300 is equipped with a blue laser module (wavelength of 488 nm).
Both the Nanoparticle Tracking analysis NS300 and Nanosight Sample Assistant were extensively used for samples containing Virus-like particles (VLPs) with sizes between 100 to 200 nm.

Zuordnung zur Core Facility

Austrian Centre of Industrial Biotechnology (acib)

Dr. Martin Trinker
Director Business Development & Fundraising
+43 316 873 9316
martin.trinker@acib.at
http://www.acib.at
The device can be used in research collaborations. Costs depend on duration of service.
The device is used in a cooperation project with a company partner in the field of vaccine development.
Fuenmayor, J., Cervera, L., Gòdia, F., & Kamen, A. (2019). Extended gene expression for Gag VLP production achieved at bioreactor scale. Journal of Chemical Technology & Biotechnology, 94(1), 302-308.

González-Domínguez, I., Puente-Massaguer, E., Cervera, L., & Gòdia, F. (2020). Quality Assessment of Virus-Like Particles at Single Particle Level: A Comparative Study. Viruses, 12(2), 223.

Pereira Aguilar, P., González‐Domínguez, I., Schneider, T. A., Gòdia, F., Cervera, L., & Jungbauer, A. (2019). At‐line multi‐angle light scattering detector for faster process development in enveloped virus‐like particle purification. Journal of separation science, 42(16), 2640-2649.

Aguilar, P. P., Schneider, T. A., Wetter, V., Maresch, D., Ling, W. L., Tover, A., ... & Jungbauer, A. (2019). Polymer-grafted chromatography media for the purification of enveloped virus-like particles, exemplified with HIV-1 gag VLP. Vaccine, 37(47), 7070-7080.

Reiter, K., Aguilar, P. P., Wetter, V., Steppert, P., Tover, A., & Jungbauer, A. (2019). Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography. Journal of Chromatography A, 1588, 77-84.