Forschungsinfrastruktur

Vienna Biocenter Core Facilities GmbH (VBCF)

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Kurzbeschreibung

The goal of our Core Facility is to provide cutting edge next generation DNA sequencing technology to its users. Currently, sequencing is performed on Illumina, PacBio and Nanopore Sequencing platforms.

Advice and guidance of sequencing projects are offered.

Depending on the required application, library preparation can either be performed by the users or by the NGS team. Multiplexed, barcoded samples can be used to fully exploit the sequencing capacity of the systems.

Base calling and primary analysis are standardly applied to the sequence reads resulting in bam/fastq files and quality information.

Our Core Facility team set up a selection of bioinformatics tools in order to assist you in further data analysis.

Ansprechperson

Andreas Sommer

Research Services

All common sequencing applications (ChIP-seq, RNA-seq, Whole Genome Sequencing etc.) are supported by
- Advice and guidance of sequencing projects
- Library preparation incl. multiplexing
- Sequencing
- Base calling and primary analysis
- Long Read Sequencing (PacBio)

Methoden & Expertise zur Forschungsinfrastruktur

All common sequencing applications (ChIP-seq, RNA-seq, Whole Genome Sequencing etc.) are supported and the development of novel methods and protocols encouraged.

Equipment der Core Facility

• DNA Sequencer III
• Pacific Biosciences Sequel System
• Biomek i7 Hybrid
• Next Seq 550 Sequencing
• NovaSeq 6000 Sequencing System