Medizinische Universität Wien
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The maXis Impact q-ToF mass spectrometer is a very sensitive and precise instrument.
The mass spectrometer is used most of the time with the Captive Spray ion-source due to a higher spray stability and higher sensitivity (down to 50 fMol of digested BSA). The mass spectrometer operates with a mass precision of 0.1 ppm and uses external standard fro re-calibration.
The system can be operated very easy and the software control is intuitive.
Peptide mapping upon nano HPLC separation, mass measurement of intact proteins. Protein and peptide qunatitation.
Methoden & Expertise zur Forschungsinfrastruktur
The Proteomics Core facility at the Medical University of Vienna employs different methods for sample preparation and analysis. While general protein extraction and enzymatic digestion methods are used according to default protocols, the separation of analytes is performed by applying dedicated methods developed in the Facility.
Different chromatographic systems and approaches are used depending on sample type and questions asked. Proteins, peptides and small molecules are being separated using ion-exchange, reversed phase, immuno-affinity, hydrophilic and hydrophobic chromatography.
Generally, all separation approaches can be coupled to a mass spectrometer for signal detection and analysis. The maXis q-ToF mass spectrometer enables detection and analysis of peptides and proteins with high mass accuracy.