Universität Salzburg
Salzburg | Website
Großgerät
Kurzbeschreibung
Quadrupol-Orbitrap Massenspektrometer für den Einsatz in der Proteom- und Metabolom-Analyse, Probeneinlass durch Direktinfusion mit Spritzenpume oder HPLC, ESI-Quelle, Nano-ESI-Quelle, durch speziellen Protein-Mode besonders geeignet für intakte Protein Messungen, zusätzlich Hochauflösungs-Option bis 250.000
Ansprechperson
Prof. Dr. Christian Huber
Research Services
Hochempfindliche Detektion und Identifizierung von Biomolekülen
Methoden & Expertise zur Forschungsinfrastruktur
Das Gerät wird zur hochempfindlichen Detektion und Identifizierung von Biomolekülen anhand der akkuraten Masse oder anhand von Fragmentierungsspektren verwendet. Mit Hilfe solcher Messungen können z. B. Expressionsprofile von Proteinen oder Abbauprodukte von Medikamenten analysiert werden.
Zuordnung zur Core Facility
Prof. Dr. Christian Huber
Fachbereich Biowissenschaften
0043 668 8044 5738
c.huber@sbg.ac.at
https://www.plus.ac.at/biowissenschaften/
Fachbereich Biowissenschaften
0043 668 8044 5738
c.huber@sbg.ac.at
https://www.plus.ac.at/biowissenschaften/
Bitte kontaktieren Sie uns unter science.plus@sbg.ac.at, oder kontaktieren Sie direkt die/den FI-Verantwortliche/n
Sandoz Biopharmaceuticals, Kundl, im Rahmen des Christian Doppler Labors für Biosimilar Charakterisierung
Predict-IV – Profiling the toxicity of new drugs; a non animal-based approach integrating toxicodynamics and biokinetics
2008-2013
Wolfgang Dekant, Koordinator
Commission of the European Communities
http://www.predict-iv.toxi.uni-wuerzburg.de/
MARINA: Managing the risks of Nanoparticles
2008-2013
Lang Tran, Koordinator
Commission of the European Communities
http://www.marina-fp7.eu/
Doktoratskolleg Immunity in Cancer and Allery; W 1213
2008-2017
Josef Thalhammer, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
2013-2020
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
2008-2013
Wolfgang Dekant, Koordinator
Commission of the European Communities
http://www.predict-iv.toxi.uni-wuerzburg.de/
MARINA: Managing the risks of Nanoparticles
2008-2013
Lang Tran, Koordinator
Commission of the European Communities
http://www.marina-fp7.eu/
Doktoratskolleg Immunity in Cancer and Allery; W 1213
2008-2017
Josef Thalhammer, Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
2013-2020
Christian Huber, Laborleiter
Christian Doppler Forschungsgesellschaft
Application of integrated transcriptomic, proteomic and metabolomic profiling for the delineation of mechanisms of drug induced cell stress
2013
Wilmes A., Limonciel A., Aschauer L., Moenks K., Bielow C., Leonard M.O., Hamon J., Carpi D., Ruzek S., Handler A., Schmal O., Herrgen K., Bellwon P., Burek C., Truisi G.L., Hewitt P., Di Consiglio E., Testai E., Blaauboer B.J., Guillou C., Huber C.G., Lukas A., Pfaller W., Mueller S.O., Bois F.Y., Dekant W., Jennings P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022
Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
2014
Behmüller R., Forstenlehner I. C., Tenhaken R., Huber C.G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3
Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry
2013
Neu V., Bielow C., Gostomski I., Wintringer R., Braun R., Reinert K., Schneider P., Stuppner H., Huber C.G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j
Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
2015
Ranninger C., Rurik M., Limonciel A., Ruzek S., Reischl R., Wilmes A., Jennings P., Dekant W., Kohlbacher O., Huber C.G.
Journal of Biological Chemistry
http://www.jbc.org/cgi/doi/10.1074/jbc.M115.644146
Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
2015
Forstenlehner I., Holzmann J., Toll H., Huber C.G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029
Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
2015
Boyles M.S.P., Kristl T., Andosch A., Zimmermann M., Tran N., Casals E., Himly M., Puntes V., Huber C.G., Lütz-Meindl U., Duschl A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9
2013
Wilmes A., Limonciel A., Aschauer L., Moenks K., Bielow C., Leonard M.O., Hamon J., Carpi D., Ruzek S., Handler A., Schmal O., Herrgen K., Bellwon P., Burek C., Truisi G.L., Hewitt P., Di Consiglio E., Testai E., Blaauboer B.J., Guillou C., Huber C.G., Lukas A., Pfaller W., Mueller S.O., Bois F.Y., Dekant W., Jennings P.
Journal of Proteomics
DOI: dx.doi.org/10.1016/j.jprot.2012.11.022
Quantitative HPLC-MS Analysis of Nucleotide Sugars in Plant Cells following Off-Line SPE Sample Preparation
2014
Behmüller R., Forstenlehner I. C., Tenhaken R., Huber C.G.
Analytical and Bioanalytical Chemistry
DOI: 10.1007/s00216-014-7746-3
Rapid and Comprehensive Impurity Profiling of Synthetic Thyroxine by Ultrahigh-Performance Liquid Chromatography−High-Resolution Mass Spectrometry
2013
Neu V., Bielow C., Gostomski I., Wintringer R., Braun R., Reinert K., Schneider P., Stuppner H., Huber C.G.
Analytical Chemistry
DOI: dx.doi.org/10.1021/ac303722j
Nephron Toxicity Profiling via Untargeted Metabolome Analysis Employing a High-Performance Liquid Chromatography-Mass Spectrometry-Based Experimental and Computational Pipeline
2015
Ranninger C., Rurik M., Limonciel A., Ruzek S., Reischl R., Wilmes A., Jennings P., Dekant W., Kohlbacher O., Huber C.G.
Journal of Biological Chemistry
http://www.jbc.org/cgi/doi/10.1074/jbc.M115.644146
Site-Specific Characterization and Absolute Quantification of Pegfilgrastim Oxidation by Top-Down High-Performance Liquid Chromatography-Mass Spectrometry
2015
Forstenlehner I., Holzmann J., Toll H., Huber C.G.
Analytical Chemistry
DOI: 10.1021/acs.analchem.5b02029
Chitosan functionalisation of gold nanoparticles encourages particle uptake and induces cytotoxicity and pro-inflammatory conditions in phagocytic cells, as well as enhancing particle interactions with serum components. Journal of Nanobiotechnology
2015
Boyles M.S.P., Kristl T., Andosch A., Zimmermann M., Tran N., Casals E., Himly M., Puntes V., Huber C.G., Lütz-Meindl U., Duschl A.
Journal of Proteomics
DOI: 10.1186/s12951-015-0146-9