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State of the art true confocal system, equipped with 4 lasers (Diode, Argon, DPSS, Helium/Neon) giving 8 excitation lines (405, 458, 476, 488, 496, 514, 561, 633 nm), conventional and resonant scanner for fast acquisition speed, acousto-optical beam splitter (AOBS) for better transmission of the emitted light, acousto-optical tunable filter (AOTF) for fine control of laser power, 3 photomultipliers (PMTs) with spectral detectors for free choice of the emission window and one hybrid detector (hyD) for optimal signal-to-noise ratio acquisition in the red emission region, 5 objectives (10x, 20x, 40x and two 63x) including one 63x water immersion objective for imaging of living samples, temperature, humidity and CO2 control. The confocal microscope is housed in a biosafety level II laboratory room equipped with one biosafety level II hood, one cell culture incubator, fridge, freezer, one small fume hood, one system producing type III deionized water.
Priv.-Doz. Silvia Dossena, PhD
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Methoden & Expertise zur Forschungsinfrastruktur
Multilabel imaging, immunocytochemistry, quantitative fluorescence, time lapse, co-localization, fluorescence resonance enery transfer (FRET), fluorescence recovery after bleaching (FRAP).
De Moraes VC, Bernardinelli E, Zocal N, Fernandez JA, Nofziger C, Castilho AM, Sartorato EL, Paulmichl M, Dossena S. Reduction of cellular expression levels is a common feature of functionally affected pendrin (SLC26A4) protein variants. Mol Med. 2016 Jan 4. doi: 10.2119/molmed.2015.00226. [Epub ahead of print] PubMed PMID: 26752218; PubMed Central PMCID: PMC5004711.
Pera A, Dossena S, Rodighiero S, Gandía M, Bottà G, Meyer G, Moreno F, Nofziger C, Hernández-Chico C, Paulmichl M. Functional assessment of allelic variants in the SLC26A4 gene involved in Pendred syndrome and nonsyndromic EVA. Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18608-13. doi: 10.1073/pnas.0805831105. Epub 2008 Nov 18. PubMed PMID: 19017801; PubMed Central PMCID: PMC2584577.