Patch clamp set-up IV - Electrophysiology Facility

Paracelsus Medizinische Privatuniversität Salzburg

Salzburg | Website

Großgerät

Kurzbeschreibung

State of the art patch-clamp set-up composed of an EPC 10 USB amplifier (HEKA, Germany), pneumatic anti-vibrating table, piezoelectric micromanipulator (Scientifica), inverted phase contrast microscope (Motic AE31) equipped with epi-fluorescence and FITC and TRITC emission filters for identification of transfected cells, perfusion system with ALA VC4 valve commander and vacuum pump, Macintosh computer running the PatchMaster acquisition software. Users will have access to a horizontal puller (Sutter Instruments) and a microforge (Narishige) for construction of electrodes and to a biosafety level II cell culture room.

Ansprechperson

Priv.-Doz. Silvia Dossena, PhD

Research Services

Please get in touch with the contact persons.

Methoden & Expertise zur Forschungsinfrastruktur

Patch-clamp recordings of currents of chloride, sodium, calcium or potassium ions on mam-malian cells, mainly in whole-cell configuration. In principle, also single-channel recordings are possible.

Priv.-Doz. Silvia Dossena, PhD
+43 (0)662 2420 80564
silvia.dossena@pmu.ac.at

Univ.-Prof. Dr. Wolfgang Patsch
+43 (0)662 2420 80560
wolfgang.patsch@pmu.ac.at
Please get in touch with the contact persons.
Morabito R, Costa R, Rizzo V, Remigante A, Nofziger C, La Spada G, Marino A, Paulmichl M, Dossena S. Crude venom from nematocysts of Pelagia noctiluca(Cnidaria: Scyphozoa) elicits a sodium conductance in the plasma membrane of
mammalian cells. Sci Rep. 2017 Jan 23;7:41065. doi: 10.1038/srep41065. PubMed PMID: 28112211; PubMed Central PMCID: PMC5253680.

Tamma G, Dossena S, Nofziger C, Valenti G, Svelto M, Paulmichl M. EGF stimulates IClswell by a redistribution of proteins involved in cell volume regulation. Cell Physiol Biochem. 2011;28(6):1191-202. doi: 10.1159/000335851. Epub 2011 Dec 16. PubMed PMID: 22179007.

Kössler S, Nofziger C, Jakab M, Dossena S, Paulmichl M. Curcumin affects cell survival and cell volume regulation in human renal and intestinal cells. Toxicology. 2012 Feb 26;292(2-3):123-35. doi: 10.1016/j.tox.2011.12.002. Epub
2011 Dec 9. PubMed PMID: 22178266; PubMed Central PMCID: PMC3274693.

Dossena S, Gandini R, Tamma G, Vezzoli V, Nofziger C, Tamplenizza M, Salvioni E, Bernardinelli E, Meyer G, Valenti G, Wolf-Watz M, Fürst J, Paulmichl M. The molecular and functional interaction between ICln and HSPC038 proteins modulates the regulation of cell volume. J Biol Chem. 2011 Nov 25;286(47):40659-70. doi: 10.1074/jbc.M111.260430. Epub 2011 Sep 14. PubMed PMID: 21917931; PubMed Central PMCID: PMC3220508.

Gandini R, Dossena S, Vezzoli V, Tamplenizza M, Salvioni E, Ritter M, Paulmichl M, Furst J. LSm4 associates with the plasma membrane and acts as a co-factor in cell volume regulation. Cell Physiol Biochem. 2008;22(5-6):579-90. doi: 10.1159/000185542. Epub 2008 Dec 9. PubMed PMID: 19088440.

Garavaglia ML, Bononi E, Dossena S, Mondini A, Bazzini C, Lanata L, Balsamo R, Bagnasco M, Conese M, Bottà G, Paulmichl M, Meyer G. S-CMC-Lys protective effects on human respiratory cells during oxidative stress. Cell Physiol Biochem. 2008;22(5-6):455-64. doi: 10.1159/000185494. Epub 2008 Dec 9. PubMed PMID: 19088427.

Guizzardi F, Rodighiero S, Binelli A, Saino S, Bononi E, Dossena S, Garavaglia ML, Bazzini C, Bottà G, Conese M, Daffonchio L, Novellini R, Paulmichl M, Meyer G. S-CMC-Lys-dependent stimulation of electrogenic glutathione secretion by human respiratory epithelium. J Mol Med (Berl). 2006 Jan;84(1):97-107. Epub 2005 Nov 11. PubMed PMID: 16283140.

Dossena S, Maccagni A, Vezzoli V, Bazzini C, Garavaglia ML, Meyer G, Fürst J, Ritter M, Fugazzola L, Persani L, Zorowka P, Storelli C, Beck-Peccoz P, Bottà G, Paulmichl M. The expression of wild-type pendrin (SLC26A4) in human embryonic kidney (HEK 293 Phoenix) cells leads to the activation of cationic currents. Eur J Endocrinol. 2005 Nov;153(5):693-9. PubMed PMID: 16260428.

Meyer G, Rodighiero S, Guizzardi F, Bazzini C, Bottà G, Bertocchi C, Garavaglia L, Dossena S, Manfredi R, Sironi C, Catania A, Paulmichl M. Volume-regulated Cl- channels in human pleural mesothelioma cells. FEBS Lett. 2004 Feb 13;559(1-3):45-50. PubMed PMID: 14960305.