BD FACSCanto II Zellscanner

Universität Salzburg

Salzburg | Website

Großgerät

Kurzbeschreibung

Durchflusszytometer BD FACS Canto II zur quantitativen und qualitativen Charakterisierung von Zellpopulationen. 3-Lasersystem (405nm, 488nm, 633nm) zur gleichzeitigen Charakterisierung von bis zu 8 Farben + FSC und SSC

Ansprechperson

Assoz. Prof. Dr. Richard Weiss

Research Services

Analyse von Zellen mittels Durchflusszytometrie

Methoden & Expertise zur Forschungsinfrastruktur

Das Gerät ist das einzige dieser Art an der gesamten Naturwissenschaftlichen Fakultät und wird von allen immunologisch forschenden Arbeitsgruppen verwendet. Flowzytometrie ist eine Grundvoraussetzung für state-of-the-art Immunologie und für die meisten zellulär-immunologischen Arbeiten, z.B. in der Tumor- oder Allergieforschung.

Zuordnung zur Core Facility

Durchflusszytrometrie

Assoz. Prof. Dr. Richard Weiss
Fachbereich Biowissenschaften, Schwerpunkt ACBN
0043 662 8044 5737
richard.weiss@sbg.ac.at
http://www.uni-salzburg.at/index.php?id=77&MP=77-44794
Bitte um Kontaktaufnahme mit der Universität Salzburg (science_plus@sbg.ac.at) oder mit der/dem FI-Verantwortlichen.
Fachbereich Zellbiologie, Universität Salzburg
Fachbereich Ökologie und Evolution, Universität Salzburg
Immunity in cancer and allergy
2008-2017
Josef Thalhamer
FWF Doktoratskolleg W1213

Influence of fold-stability on allergenicity and immunogenicity
2014-2017
Richard Weiss
FWF P26997-B13

P.L.E.A.S.E. Vaccinate
2015
Richard Weiss
Pantec Biosolutions, Liechtenstein

Immune functions of epidermal Langerhans cells in vivo
2013-2016
Angelika Stöcklinger
FWF P25243-B22

Signaling crosstalk between NOD2 and IL-31
2013-2016
Jutta Horejs-Höck
FWF P25696

IL-31 regulates DC-mediated T cell responses in the course of atopic dermatitis
2011-2015
Jutta Horejs-Höck
FWF P23933

Immune regulation in the skin and induction of immunological tolerance
2014-2017
Iris Gratz
Debra Austria

Activation and maintenance of effector and regulatory T cells in the skin
2013-2016
Iris Gratz
R03AR064554 National Institute of Health (NIH), USA

The interplay between tree pollen allergens and their sources
2015-2018
Fatima Ferreira
FWF P27589

An innovative causal therapy for allergy: safe and rapid induction of an anti-inflammatory immune response using a mutant hypoallergen and vitamin D3
2014-2019
Fatima Ferreira, EU-FP7-Health-2013-Innovation-1 GA 601763

Mapping antibody profiles of Bet v 1 and associated food allergens during SIT
2014-2016
Michael Wallner
FWF P26125-B13

Immunomodulatory Effects of Ligand-binding to Pollen Allergens
2011-2016
Michael Wallner
FWF P23417-B13

Hedgehog – Interleukin 6 Signal Cooperation in Basal Cell Carcinoma
2013-2016
Fritz Aberger
FWF P25629
Chitosan Functionalisation of Gold Nanoparticles Encourages Particle Uptake and Induces Cytotoxicity and Pro-Inflammatory Conditions in Phagocytic Cells, as Well as Enhancing Particle Interactions with Serum Components
2015
Boyles, M. S., T. Kristl, A. Andosch, M. Zimmermann, N. Tran, E. Casals, M. Himly, V. Puntes, C. G. Huber, U. Lutz-Meindl and A. Duschl
J Nanobiotechnology 13, 84

Prophylactic Mrna Vaccination against Allergy Confers Long-Term Memory Responses and Persistent Protection in Mice
2015
Hattinger E., S. Scheiblhofer E. Roesler, T. Thalhamer, J. Thalhamer and R. Weiss
J Immunol Res, 797421

Fold Stability During Endolysosomal Acidification Is a Key Factor for Allergenicity and Immunogenicity of the Major Birch Pollen Allergen
2015
Machado Y., R. Freier, S. Scheiblhofer, T. Thalhamer, M. Mayr, P. Briza, S. Grutsch, L. Ahammer, J. E. Fuchs, H. G. Wallnoefer, A. Isakovic, V. Kohlbauer, A. Hinterholzer, M. Steiner, M. Danzer, J. Horejs-Hoeck, F. Ferreira, K. R. Liedl, M. Tollinger, P. Lackner, C. M. Johnson, H. Brandstetter, J. Thalhamer and R. Weiss
J Allergy Clin Immunol.

Immune Reactions against Gene Gun Vaccines Are Differentially Modulated by Distinct Dendritic Cell Subsets in the Skin
2015
Weber C. S., K. Hainz, T. Deressa, H. Strandt, D. Florindo Pinheiro, R. Mittermair, J. Pizarro Pesado, J. Thalhamer, P. Hammerl and A. Stoecklinger
PLoS One 10, no. 6 e0128722

Hax1 Deletion Impairs Bcr Internalization and Leads to Delayed BcrMediated Apoptosis
2015
Wolkerstorfer S., E. Schwaiger, M. Rinnerthaler, I. Karina Gratz, T. Zoegg, H. Brandstetter and G. Achatz-Straussberger
Cell Mol Immunol

Hedgehog/Gli and Pi3k Signaling in the Initiation and Maintenance of Chronic Lymphocytic Leukemia
2015
Kern D., G. Regl, S. W. Hofbauer, P. Altenhofer, G. Achatz, A. Dlugosz, H. Schnidar, R. Greil, T. N. Hartmann and F. Aberger Oncogene

Residual Endotoxin Contaminations in Recombinant Proteins Are Sufficient to Activate Human Cd1c+ Dendritic Cells
2014
Schwarz H., M. Schmittner, A. Duschl and J. Horejs-Hoeck
PLoS One 9, no. 12 e113840

The Impact of Nitration on the Structure and Immunogenicity of the Major Birch Pollen Allergen Bet V 1.0101
2014
Ackaert C., S. Kofler, J. Horejs-Hoeck, N. Zulehner, C. Asam, S. von Grafenstein, J. E. Fuchs, P. Briza, K. R. Liedl, B. Bohle, F. Ferreira, H. Brandstetter, G. J. Oostingh and A. Duschl
PLoS One 9, no. 8 e104520

Structural Integrity of the Antigen Is a Determinant for the Induction of THelper Type-1 Immunity in Mice by Gene Gun Vaccines against E. Coli Beta-Galactosidase
2014
Deressa T., A. Stoecklinger, M. Wallner, M. Himly, S. Kofler, K. Hainz, H. Brandstetter, J. Thalhamer and P. Hammerl
PLoS One 9, no. 7 e102280

Human Th2 but Not Th9 Cells Release Il-31 in a Stat6/Nf-Kappab-Dependent Way
2014
Maier E., D. Werner, A. Duschl, B. Bohle and J. Horejs-Hoeck
J Immunol.