AutoDG PCR-System Anlage

University of Innsbruck

Innsbruck | Website

Large equipment

Short Description

The QX200 AutoDG Droplet Digital PCR System simplifies the Droplet Digital PCR (ddPCR™) workflow, reducing hands-on time and eliminating user-to-user variability. The AutoDG Instrument brings scalability and flexibility to Bio-Rad’s ddPCR technology, the most precise and sensitive digital PCR solution for the absolute quantification of target DNA molecules.

AutoDG Instrument:
Guides setup and loading with large, color touch-screen interface and LED lighting
Compatible with both the QX100™ and QX200™ Droplet Digital™ PCR Systems
Generates droplets for 96 ddPCR reactions in under 45 minutes
One AutoDG Instrument can supply 4–5 droplet readers continuously
HEPA-filtered enclosure reduces contamination and allows the system to be placed in any lab environment
User-definable plate layout with increments of 8 wells provides run-to-run flexibility

Droplet Digital PCR Technology:

Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
Droplet partitioning by the QX200 Droplet Digital PCR system reduces bias from amplification efficiency and PCR inhibitors
Convenient assay design — standard curves are not required

Contact Person

Univ.-Prof. Mag. Dr. Michael Traugott

Research Services

Momentan keine Research Services für dieses Gerät.
Die Forschungsinfrastruktur ist "Open for Collaboration". Kommerzielle Kooperationen sind nicht möglich.

Methods & Expertise for Research Infrastructure

Expertise bzw. Einsatz des Systems besteht im Bereich der Quantifizierung von DNA in Umweltproben (z.B. Wasser, Boden, Nahrungsproben), insbesondere wenn DNA-Konzentrationen sehr gering sind.

Nutzung über Kooperationsprojekte im Forschungsbereich.
Nutzungsbedingungen werden im Rahmen einer wissenschaftlichen Kooperation definiert. Keine kommerzielle Nutzung möglich. Bei Interesse an einer Kooperation oder Zusammenarbeit bitten wir Sie um Kontaktaufnahme.
Zoologie (u.a.)
- FFG Bridge-1 Projekt "eDNA-AlpFisch" (Traugott)
- FFG Bridge-1 Projekt "VectorDetect" (Traugott)
- FWF Projekt "Assessing food web dynamics to improve biocontrol of pests" (Traugott)

Limnologie
- FWF-Projekt "Chemolithoautotrophe Kohlenstoffdioxid-Fixierung in Seen" (Alfreider)
- FWF-Projekt "Mechanismen und Signifikanz von Genomgrößenvariation bei Rotatorien" (Stelzer)

Mikrobiologie (u.a.)
- Euregio IPN 94-B29 (Präg)
- FWF-Projekt „Luftdruck-Effekte auf höher wandernde alpine Ökosysteme“ (I 5241, Illmer)
u.a.:

Alfreider A, Tartarotti B. Spatiotemporal dynamics of different CO2 fixation strategies used by prokaryotes in a dimictic lake. Sci Rep. 2019; 9:15068. doi: 10.1038/s41598-019-51584-0.

Harringer M, Alfreider A. Primer evaluation and development of a droplet digital PCR protocol targeting amoA genes for the quantification of Comammox in lakes. Sci Rep. 2021; 11:2982. doi: 10.1038/s41598-021-82613-6.

Praeg, Nadine; Schachner, Iris; Schuster, Lisa; Illmer, Paul (2021): Carbon-dependent growth, community structure, and methane oxidation performance of a soil-derived methanotrophic mixed culture.
In: FEMS Microbiology Letters 368/2, No. fnaa212. (DOI) (Weblink)

Praeg, Nadine; Illmer, Paul (2020): Microbial community composition in the rhizosphere of Larix decidua under different light regimes with additional focus on methane cycling microorganisms.
In: Scientific Reports 10, No. 22324. (Volltext) (DOI) (Weblink)

Thalinger B., Pütz Y. & Traugott M. (2021): Endpoint PCR coupled with capillary electrophoresis (celPCR) provides sensitive and quantitative measures of environmental DNA in singleplex and multiplex reactions. PlosOne, 16(7): e0254356, https://doi.org/10.1371/journal.pone.0254356

Thalinger B., Rieder A., Teuffenbach A., Pütz Y., Schwerte T., Wanzenböck J. & Traugott M. (2021): The effect of activity, energy use, and species identity on environmental DNA shedding of freshwater fish. Frontiers in Ecology and Evolution, doi: 10.3389/fevo.2021.623718