Short Description
The BioIndustrial Pilotplant at BOKU was engineered according to GMP guidelines and comprises a modular fermentation plant for cultivation of mamalian and microbial cells on industrial scale.
For purification of products a disc stack separator, centrifuges, high pressure homogenizer, depth-, ultra- and dia-filtration units, and chromatographic systems are available.
Contact Person
DI Dr. Markus Luchner
Research Services
* Process development for up- and downstream processing with different host organism (bacterial, yeast, cell
culture,…)
* Fermentation scale up from µ- to lab- and pilot scale
* On-line monitoring platform and development of monitoring strategies for critical control points in real-time in
up- and downstream
* Manufacturing and processing of products at pilot scale (non-GMP, but under “GMP-like” conditions)
* Available in-house model systems (e.g. rhSOD, GFP, scFv (IB or soluble)) and model organisms (e.g. E. coli,
P. pastoris, CHO, Sf9)
* Open for model systems and organisms provided by partners
* Method development for cell harvest, IB harvest, refolding, precipitation; small scale development and transfer
to pilot scale
* Filter testing for Depth- and Sterile filtration including upscale
* Ultra-/Diafiltration, method development and upscale; membrane testing
* Chromatographic separation; method development and upscale; benchmarking of different resins with in-house
model systems (different feed)
* Test of equipment provided by supplier
Methods & Expertise for Research Infrastructure
* Process development for up- and downstream processing with different host organism (bacterial, yeast, cell culture,…)
* Fermentation scale up from µ- to lab- and pilot scale
* On-line monitoring platform and development of monitoring strategies for critical control points in real-time in up- and downstream
* Manufacturing and processing of products at pilot scale (non-GMP, but under “GMP-like” and "high quality" conditions)
* Available in-house model systems (e.g. rhSOD, GFP, scFv (IB or soluble)) and model organisms (e.g. E. coli, P. pastoris, CHO, Sf9)
* Open for model systems and organisms provided by partners
* Method development for cell harvest, IB harvest, refolding, precipitation; small scale development and transfer to pilot scale
* Filter testing for Depth- and Sterile filtration including upscale
* Ultra-/Diafiltration, method development and upscale; membrane testing
* Chromatographic separation; method development and upscale; benchmarking of different resins with in-house model systems (different feed)
* Test of equipment provided by supplier
For further information please contact Dr. Markus Luchner (markus.luchner@boku.ac.at).
Univeristy of Applied Sciences Vienna
Industrial companies