LSM 700 (confocal)

Medical University of Vienna

Wien | Website

Large equipment

Short Description

The LSM 700 is an inverted point laser scanning microscope. It is equipped with 4 solid state laser. This microscope has only one pinhole, which is fully motorized and can be automatically positioned depending on illumination and detection wavelength. This is especially important in multifluorescence experiments guaranteeing the optimal section thickness for every wave lenght. Furthermore the system supports spectral imaging by a special beam splitter dividing the emitted light with 1nm steps between two detectors. The microscope is very easy to handle and very popular under our users.

The system supports following applications (- or combinations thereof):
- Imaging of fixed probes (tissue, cells)
- Z-Staple (3D)
- Time Series
- Tiles
- Multiple Positions
- Tissue Scanning

Technical Data:

Wide Field Illumination:
Halogen Lamp 12V, 100W
HXP 120W
Filter Sets: DAPI, GFP, Rhodamin

Laser Lines:
Solid State Lasers:
405nm, 5mW
488nm, 10mW
555nm, 10mW
639nm, 5mW

Filters:
PMT 1: SP 490, SP 555, SP 640 nm
PMT 2: LP 490, LP 560, LP 640 nm
VSD Beamsplitter: 416-728 in 1nm Steps
Transmission Light: T-PMT

Objectives:
5x/ 0.16 Plan-Apochromat
10x/ 0.3 EC Plan-Neofluar
20x/ 0.8 Plan-Apochromat
40x/ 1.3 Plan-Apochromat, Oil, DIC
63x/ 1.4 Plan-Apochromat, Oil, DIC

Software:
ZEN 2009 black
Physiology Package (Analysis Software for Time Series, ROI –Analysis, Online- und Offline Calibration of Ion Concentrations)
Multiple Time Series
Experiment Designer Module
Tile and Stich Module
Tiles and Positions Module


Furthermore:
XYZ motorized
DIC
on request 100x / 1.4 Objective and 40x/ 1.1 LD C-Apochromat
Water Objective
Stage-Insert for 4 Slides on request

Fees:
28€ / h

Contact Person

Marion Gröger, Sabine Rauscher

Research Services

Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance

Methods & Expertise for Research Infrastructure

The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies.

The available systems support high resolution imaging of tissue sections or cells, 3D and 4D acquisition, visualization of protein-protein interactions (like FRET) and live cell imaging. Furthermore the facility offers workstations to the users, which allow an onsite evaluation and processing of their data with the suitable software.

In addition the area of microscopy the core facility houses a small histology unit for sample preparation. This is equipped with one cryostat and two microtomes. The microtomes are only to cut paraffin embedded material for the purpose of RNA and DNA extraction.

For performing live cell imaging the facility provides a fully equipped cell culture lab.

To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.

The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!

Allocation to Core Facility

Core Facility Imaging

To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.

The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!

fee (LSM 700): 28€/h