Mass Spectrometer/Bruker/timsTOF Pro 2

Vienna Biocenter Core Facilities GmbH (VBCF)

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Large equipment

Short Description

The timsTOF Pro (Bruker) is a time-of -flight instrument coupled to a dual Trapped Ion Mobility Spectrometry (TIMS) analyser. Here, ions are separated in the gas phase based on their ion mobility which adds a 3rd dimension of separation in addition to retention time and m/z. The design of the dual TIMS device allows for ions to be accumulated in the first part of the analyser, while they are sequentially released dependent on their ion mobility from the second section (PASEF; Parallel Accumulation and Serial Fragmentation). Thereby a duty cycle of nearly 100% is achieved resulting in nearly no ion loss. The design of the instrument offers a high sequencing speed (up to 100Hz) by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel. This design allows to reach new depths in shotgun proteomics and phosphoproteomics, using low sample amounts.
MS instruments used for shotgun proteomics require cleaning when run 24 hours a day on large sample numbers, and performance degradation can be noticeable over short time periods. The superior robustness of the timsTOF Pro allows the instrument to be run 24/7 over many days or weeks without noticeable loss of sensitivity or other performance metrics.

Contact Person

Karl Mechtler

Research Services

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Allocation to Core Facility

Mass Spectrometry

Karl Mechtler
Vienna Biocenter Core Facilities (VBCF)
+43 1 79730 4280
Vienna BioCenter Core Facilities (VBCF) offer state-of-the-art research infrastructure and scientific services to academic research institutes and companies in the field of Life Sciences at Vienna BioCenter and beyond. Our general cooperation conditions are available at (Downloads – VBCF General Cooperation Conditions). Information on usage fees is provided upon request by the core facilities.