Molecular Biodiversity Lab

University of Salzburg

Salzburg | Website

Short Description

The Core Facility Molecular Biodiversity Lab processes RNA, DNA and hormone samples using molecular biological and analytical techniques, which allow describing biodiversity on a molecular level. We apply techniques of DNA-Barcoding and Metabarcoding as well as genomical and population genetical techniques.

This includes the following work steps and notable equipment:
- DNA isolation ( including incubators),
- PCR (thermocyclers and UV-workstation),
- gel electrophoresis and gel documentation, DNA purification, next generation sequencing (Covaris Ultrasonicator, Magnetic Bead Purification),
- hormone extraction (orbital shaker, vakuum centrifuge).

In addition, a reverse osmosis ultrapure water system and various centrifuges (including a refrigerated centrifuge) are available. The Core Facility Molecular Biodiversity Lab is partner of ABOL (Austrian Barcode of Life).

Contact Person

Univ.- Prof. Jan C. Habel

Research Services

DNA-Isolation, single gene PCR (including DNA-Barcoding);

Methods & Expertise for Research Infrastructure

Expertises include isolation of RNA & DNA from various types of samples, PCR amplification of single genes (including DNA barcoding), fragment length analysis (microsatellite genotyping, AFLPs, RADseq), and next generation sequencing, including genome sequencing, transcriptome sequencing, and targeted gene enrichment as well as genomic and sequence based numerical analystics. Other expertise includes the extraction of steroid hormones from blood plasma and feathers and the quantification of hormone titers using enzyme immunoassays.