O2k-FluoRespirometer

Oroboros Instruments

Innsbruck | Website

Other research infrastructure

Short Description

Modular O2k-system for high-resolution respirometry and fluorometry.
Mitochondria and Cell Research.
Research areas: fundamental bioenergetics, analysis of mitochondrial and metabolic diseases, field of preventive mitochondrial medicine.
Find solutions for and study mitochondrial function in cancer, diabetes, aging, neurodegeneration, exercise physiology and mitochondrial medicine.

Research Services

OXPHOS Analysis of mitochondrial function in disease and healthy controls.

Methods & Expertise for Research Infrastructure

With the O2k-FluoRespirometer small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissue slices. It is used to simultaneously measure and monitor oxygen consumption and either ROS production (AmR), mitochondrial (mt-) membrane potential (TMRM, Safranin and Rhodamine), Ca2+ (CaG) or ATP production (MgG). The O2k-FluoRespirometer supports all add-on MultiSensor Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.

The modular O2k-Concept is supported by the specially developed O2k-Software, DatLab 7, guiding the user step-by-step through the desired experiment. In addition, international O2k-Workshops are provided on a regular basis, allowing for hands-on practical training.

Oroboros Instruments boasts over 25 years of experience and offers the leading instrument on the market in mitochondrial physiology and pathology.
The O2k-Network includes and connects over 600 reference laboratories worldwide and labs, hospitals, research centers, pharmaceutical and biotech companies in 49 countries.
The open-access preprint server, MitoFit Preprint Archives, encourages the sharing of knowledge in the field and supports our strong belief in having an open source platform for customers and network.

For further information have a look at our O2k-Top10 corporate commitment - 10 compelling reasons for the Oroboros O2k, and for collaborating with Oroboros Instruments: https://wiki.oroboros.at/images/4/44/MiPNet14.10_O2k-Top10.pdf

Contact the Oroboros-Team under
instruments@oroboros.at
Please contact instruments@oroboros.at
Oroboros Instruments closely collaborates with the Medical University of Innsbruck and research institutions world-wide.
Oroboros Instruments is involved in an extensive range of European and international research projects, for example:
- NextGen-O2k H2020 SME Phase 2
- COST Action 15203 MitoEAGLE
- Marie Sklodowska-Curie Projects TRACT and TRANSMIT
Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6.

Gnaiger E (2019) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Mitochondr Physiol Network 24.05. Oroboros MiPNet Publications, Innsbruck:112 pp.

Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38.

Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81.Lemieux H, Subarsky P, Doblander C, Wurm M, Troppmair J, Gnaiger E (2019) Mitochondrial respiratory function as an early biomarker of apoptosis induced by growth factor removal. bioRxiv doi: https://doi.org/10.1101/151480

Oemer G, Lackner L, Muigg K, Krumschnabel G, Watschinger K, Sailer S, Lindner H, Gnaiger E, Wortmann SB, Werner ER, Zschocke J, Keller MA (2018) The molecular structural diversity of mitochondrial cardiolipins. Proc Nat Acad Sci U S A 115:4158-63.

Further publications are accessible online:
http://www.oroboros.at