Short Description
Our Live Imaging Microscope is an inverted wide field microscope. It is equipped with an environmental control and different small incubation chambers for CO2 and humidity supply. As a sensitive and fast camera, the hamamathsu orca flash 4 sCMOS is used for image acquisition. Furthermore it offers a motorized stage, Z – drift compensation, LED light sources, DIC optics, phase contrast and filtersets for DAPI, FITC, Cy3, Cy5, CFP und YFP.
The system supports following applications (- or combinations thereof):
• Z-staple (3D)
• Time Series
• Multiple Positions
• Tiles
• Live Cell Imaging
• FRET- Acquisition
Technical Data
Wide field Illumination:
• LED
• Filter DAPI, GFP, Cy3, Cy5, CFP, YFP
Camera:
• Orca Flash4 V2 sCMOS
Objectives:
• 4x/ 0.13 U Plan-Semi-Apochromat, Phase
• 10x/ 0.3 C Plan-FL, Phase
• 20x/ 0.7 UPC-Plan Fluor, Phase
• 40x/ 1.35 UPlan-Apochromat, Oil, DIC
• 40x/ 0.95 UPlanS Apochromat
• 60x/ 1.42 Plan-Apochromat, Oil, DIC
• 100x/ 1.4 PlanS-Apochromat, Oil, DIC
Software:
• CellSens Dimension V1
• Well Navigator
Further More:
• XYZ motorisiert
• Z-Drift-Kompensator
• Environmental control, CO2, Humidity
• Vergrößerungswechsler
• Slides, Chamber Slides
• Plates
• Petridishes (35mm)
Contact Person
Sabine Rauscher
Research Services
Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance
Methods & Expertise for Research Infrastructure
The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies. For performing live cell imaging the facility provides a fully equipped cell culture lab.
Allocation to research infrastructure
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!