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The OMNISEC multi-detector GPC/SEC system is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system and combines analytical SEC with an integrated triple-detector (Refractive Index, Light Scattering and UV/VIS PDA) to accurately determine the molecular weights and concentrations of proteins. Moreover, it can be used to analyze the purity of a sample, to determine the oligomeric state of proteins and protein complexes and to quantify the different species present in a sample. Thus, it is a powerful tool for taking control of the performance, quality and value of your protein and other biomolecules.
Dr. Elisabeth Laurent
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Methods & Expertise for Research Infrastructure
Size-exclusion chromatography is a technique in which solute molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in a column. The OMNISEC multi-detector GPC/SEC system is composed of a pump, a temperature controlled autosampler and a column oven. As the sample elutes, it is analyzed by three detectors in series. The refractive index (RI) detector determines the concentration of a sample by measuring the RI difference between a reference fluid and a sample fluid. The diode-array based UV/Vis spectrometer records the absorbance of a sample at any wavelength from 190–900 nm. The light scattering detector combines a 90° Right-Angle Light Scattering (RALS) with a 7° Low-Angle Light Scattering (LALS). While the RALS detector provides high sensitivity for smaller molecules, the LALS detector is more accurate for larger molecules. By combining both detectors into a hybrid system, the signal-to-noise ratio can be maximized for accurate determination of the molecular weight of the molecules present in a sample.