OMNISEC multi-detector GPC/SEC system

University of Natural Resources and Life Sciences Vienna (BOKU)

Wien | Website

Large equipment

Short Description

The OMNISEC multi-detector GPC/SEC system is a Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) system and combines analytical SEC with an integrated triple-detector (Refractive Index, Light Scattering and UV/VIS PDA) to accurately determine the molecular weights and concentrations of proteins. Moreover, it can be used to analyze the purity of a sample, to determine the oligomeric state of proteins and protein complexes and to quantify the different species present in a sample. Thus, it is a powerful tool for taking control of the performance, quality and value of your protein and other biomolecules.

Contact Person

Dr. Elisabeth Laurent

Research Services

Please contact the Core Facility via bmca@boku.ac.at

Methods & Expertise for Research Infrastructure

Size-exclusion chromatography is a technique in which solute molecules are separated according to their hydrodynamic radius as they enter and exit the pores of a porous gel packing matrix in a column. The OMNISEC multi-detector GPC/SEC system is composed of a pump, a temperature controlled autosampler and a column oven. As the sample elutes, it is analyzed by three detectors in series. The refractive index (RI) detector determines the concentration of a sample by measuring the RI difference between a reference fluid and a sample fluid. The diode-array based UV/Vis spectrometer records the absorbance of a sample at any wavelength from 190–900 nm. The light scattering detector combines a 90° Right-Angle Light Scattering (RALS) with a 7° Low-Angle Light Scattering (LALS). While the RALS detector provides high sensitivity for smaller molecules, the LALS detector is more accurate for larger molecules. By combining both detectors into a hybrid system, the signal-to-noise ratio can be maximized for accurate determination of the molecular weight of the molecules present in a sample.

Allocation to Core Facility

Biomolecular & Cellular Analysis (BmCA)

Dr. Elisabeth Laurent
Biomolecular & Cellular Analysis
+43 1 47654 35012
bmca@boku.ac.at
https://boku.ac.at/cf/bmca
Please contact the Core Facility via bmca@boku.ac.at