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The Microplate Reader is a laboratory device that detects chemical, biological or physical reactions by measuring the light emitted.
Univ.-Prof. Dr. Oliver Brüggemann
The system can detect classical absorption, luminescence and fluorescence, and perform more specialized fluorescence measurements, such as time-resolved fluorescence (TRF) and fluorescence polarization (FP).
Applications and research:
- Cell Migration Assays
- Cell viability
- Cytotoxicity Assay
- DNA/RNA quantification
- Enzyme kinetics
- Enzyme-Linked Immunosorbent Assays (ELISAs)
- Fluorescent proteins and FRET
- Low volume applications
- Membrane permeability
- Recording assays for neurotransmitter transporters
- Protease assays
- Protein assays
Methods & Expertise for Research Infrastructure
A microplate reader detects light emitted from samples pipetted into a microplate. The emission of light in these samples is the result of a biological, chemical, biochemical or physical reaction.