Vienna Biocenter Core Facilities GmbH (VBCF)
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Short Description
Isothermal Titration Calorimetry (ITC) is a method used to analyze biomolecular interactions by measuring the heat change that occurs when two molecules interact. Heat is liberated or absorbed as a result of the redistribution of non-covalent bonds when the interacting molecules go from the free to the bound state. The resulting isotherm is fitted to a binding model to obtain the affinity (KD), stoichiometry (N) and enthalpy of interaction (ΔH). The MicroCal PEAQ-ITC isothermal titration calorimeter from Malvern Instruments has a 200 μL sample cell and provides direct measurement of the heat absorbed or evolved as a result of mixing precise amounts of reactants in liquid samples.
Contact Person
Arthur Sedivy
Research Services
-user training
-instrument booking
-biomolecular interaction measurements as full service
Methods & Expertise for Research Infrastructure
The ProTech facility can run ITC analyses on the PEAQ-ITC instrument as a full service, or provide user training so that users can book the instrument and run their own experiments. Please contact the facility for more details.
Allocation to Core Facility
Protein Technologies Facility
+43 1 796 2324 7231
arthur.sedivy@vbcf.ac.at
https://www.vbcf.ac.at/facilities/protein-technologies/