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The 200 kV Thermo Scientific Glacios is a cryo-TEM for high-throughput sample screening and fully automated data recording. The Glacios is exclusively used for cryo-EM (single particle and cryo-tomography) and allows insight into the near-native 3D structure of biological samples up to sub-nanometer resolution.
200 kV X-FEG
Autoloader for up to 12 cryo samples
Falcon3 Direct Electron Detector
Magnetic field compensation system
The Glacios microscope is a 200 kV cryo-transmission electron microscope that is used exclusively for the analysis of cryo-samples. Such samples are typically prepared by plunge freezing and allow to study the 3D structure of biological samples as close to the native state as possible today.
Suitable Samples include:
Molecules (RNA, DNA, proteins, lipids)
Complexes (liposomes, emulsions, synthetic structures)
Parts of Eukaryotic cells
Methods & Expertise for Research Infrastructure
The Glacios is used exclusively for structural analysis of biological cryo-samples preserved as close to the native state as possible today by techniques such as plunge freezing for example.
It can be used to analyze:
molecules: proteins, lipids, nucleic acids
isolated complexes or organelles
parts of eukaryotic cells
The Glacios is used for:
high-throughput screening of cryo-samples
(automated) high-end data recording using single particle or cryo-electron tomography approaches
Allocation to Core Facility
After thorough training infrastructure users can use the Glacios microscope completely independent.
Experienced EM Facility staff will receive samples from users and perform all steps necessary for them: from sample preparation to cryo-electron microscopy to image analysis.