Vienna Biocenter Core Facilities GmbH (VBCF)
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Short Description
The OmniSEC system is an HPLC system connected to 4 detectors - UV, refractive index, right angle light scattering, and viscosity detection.
Contact Person
Arthur Sedivy
Research Services
The OmniSEC system combines analytical size exclusion chromatography with right angle light scattering and measurement of refractive index, to accurately determine protein molecular weights and concentrations. It can be used to analyze the oligomeric state of proteins and protein complexes and to quantify the different species present in a sample. A full UV-vis spectrum and intrinsic viscosity measurements enable the accurate determination of extinction coefficients and shape/size of protein samples.
Methods & Expertise for Research Infrastructure
The OmniSEC system combines analytical size exclusion chromatography with right angle light scattering and measurement of refractive index, to accurately determine protein molecular weights and concentrations. It can be used to analyze the oligomeric state of proteins and protein complexes and to quantify the different species present in a sample. A full UV-vis spectrum and intrinsic viscosity measurements enable the accurate determination of extinction coefficients and shape/size of protein samples.