Fixed samples provide only a brief snapshot of cellular processes. By observing living cells or organisms, one can gain insights into the dynamics and interaction of proteins, organelles and cells. The AxioObserver (Zeiss) is suitable for transmitted light brightfield, phase contrast, DIC, PlasDIC and incident light fluorescence and is equipped with a temperature control device and motorized object table. The microscope can be used for fluorescence-based analysis of fixed and living cells in the field of cell biology and cancer research.
Prof. Dr. Silja Wessler
Live Cell Imaging
Methods & Expertise for Research Infrastructure
The device is used in various molecular biology disciplines such as molecular tumor biology and molecular immunology research. With the help of fluorescence-based labeling methods, the device enables the analysis of fixed as well as living cells, a central technology and prerequisite for the development of highly cited publications of CF tumor biology. Equipment: Transmitted light bright field, phase contrast, DIC, PlasDIC and incident light fluorescence with a temperature control device and motorized object table
Allocation to Core Facility
Josef Thalhammer: Koordinator
Fonds zur Förderung der wissenschaftlichen Forschung
Apoptosis or Cell Survival: c-Abl in Helicobacter pylori-infected epithelial cells
Fonds zur Förderung der wissenschaftlichen Forschung, P24315
Targeting E-cadherin - Functional role of E-cadherin shedding in Helicobacter pylori-associated carcinogenesis
Fonds zur Förderung der wissenschaftlichen Forschung, P24074
Posselt G, Backert S, Wessler S
Cell Communication and Signaling
Helicobacter pylori HtrA is a new secreted virulence factor that cleaves E-cadherin to disrupt intercellular adhesion.
Hoy B, Löwer M, Weydig C, Carra G, Tegtmeyer N, Geppert T, Schröder P, Sewald N, Backert S, Schneider G, Wessler S.
CagA phosphorylation in Helicobacter pylori-infected B cells is mediated by the non-receptor tyrosine kinases of the Src and Abl families.
Krisch LM, Posselt G, Hammerl P, Wessler S
Infection and Immunity
Identification of E-cadherin signature motifs functioning as cleavage sites for Helicobacter pylori HtrA
Schmidt TP, Perna AM, Fugmann T, Böhm M, Jan Hiss, Haller S, Götz C, Tegtmeyer N, Hoy B, Rau TT, Neri D, Backert S, Schneider G, Wessler S.
E-Cadherin Orthologues as Substrates for the Serine Protease High Temperature Requirement A (HtrA)
Bernegger S, Hutterer E, Zarzecka U, Schmidt TP, Huemer M, Widlroither I, Posselt G, Skorko-Glonek J, Wessler S.