TIRF - Microscopy

Medical University of Vienna

Wien | Website

Large equipment

Short Description

Live Cell Imaging Microscope - TIRF (Total Interference Reflection Fluorescence)

Our Live Imaging Microscope is an inverted wide field microscope. It is equipped with an environmental control and different small incubation chambers for CO2 and humidity supply. As a sensitive and fast camera, the hamamathsu orca flash 4 sCMOS is used for image acquisition. Furthermore it offers a motorized stage, Z – drift compensation, LED light sources, DIC optics, phase contrast and filtersets for DAPI, FITC, Cy3, Cy5, CFP und YFP.

The TIRF-Option is based on a motorized 4Line TIRF illumination combiner.

Contact Person

Core Facility Imaging

Research Services

Advice and support in setting up protocols
Training for the equipment
Assistance in using the equipment
Data analysis

Methods & Expertise for Research Infrastructure

The Core Facility thus offers a technology for high-resolution microscopy. All processes close to the cover glass can be observed with very high resolution.

Allocation to Core Facility

Core Facility Imaging