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Microscale Thermophoresis (MST) is a new method developed to measure biomolecular interactions. The Monolith NT.115 Instrument uses fluorescence detection with either a blue (470/520) or red (625/680) filter. Labeling of a biomolecule with a fluorescent dye will enable measurement of the dissocation constant between the molecule and a variety of ligands (protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, etc).
The ProTech facility will either provide training and help with the experiment design so that you can book the instrument, or we can carry out the whole experiment for you.
Methoden & Expertise zur Forschungsinfrastruktur
Microscale Thermophoresis is a new method to cost efficiently measure molecular interactions, especially interactions of biomolecules.
General protein - ligand interactions can be measured (protein - protein, protein - DNA/RNA, protein - small molecule/ion).
The NT.115 BLUE/RED uses fluorescence as a read-out with either the BLUE filter (exc 450 em 520) or the RED filter (exc 620 em 680) therefore one of the binding partners needs to be fluorescently labeled.
Affinities as well as binding energies and stoichiometry of the molecular interaction can be determined.