Protein Technologies

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Core Facilities (CF)

Kurzbeschreibung

The Protein Technologies Facility is a core facility providing services surrounding recombinant protein production and genome engineering. We offer services in the areas of Molecular Cloning, Protein Production, Protein Purification, Protein Characterization, and CRISPR/Cas9 Genome Engineering.

Ansprechperson

Peggy Stolt-Bergner

Research Services

- Molecular Cloning
-- Stand-alone service or combined with protein production
-- Vector library with affinity tags, flourescent proteins, etc or custom tagging
-- Site-directed mutagenesis
-- Multi-gene expression constructs

- Protein Production
-- protein production in E. coli
-- protein production in insect cells using the baculovirus system
-- protein production in Drosophila S2 cells
-- protein production in HEK293 cells
-- Collaboration partners for fermentation

- Protein Purification
-- Full purification service
-- Purification training
-- Access to equipment

- Protein characterization
-- Circular Dichroism (CD)
-- Size Exclusion Chromatography coupled to Multi-Angle Laser Light Scattering (SEC-MALLS)
-- Isothermal Titration Calorimetry (ITC)
-- Dynamic Light Scattering (DLS)
-- Protein stability analysis
-- Microscale thermophoresis (MST)

- Genome Engineering
-- Consulting and assistance with experimental design
-- Generation of DNA, RNA, protein reagents
-- Assistance with protein electroporation
-- Generation of knock-out/knock-in cell lines
-- Generation of knock-out/knock-in A. thaliana

Methoden & Expertise zur Forschungsinfrastruktur

The mission of the Protein Technologies Facility (ProTech) is to further research in molecular and cell biology, protein biochemistry, and structural biology by overcoming major bottlenecks in these fields, including molecular cloning, protein production, and protein purification. We provide molecular cloning in a variety of vector systems, high quality protein production in prokaryotic and eukaryotic expression systems, protein purification, and biophysical characterization of proteins. We also provide consulting for genome engineering projects using the CRISPR/Cas9 technology, and can provide DNA, RNA, and protein reagents for CRISPR/Cas9 experiments.

Equipment der Core Facility

Peggy Stolt-Bergner
Vienna Biocenter Core Facilities (VBCF)
+43 1 7962324 7070
peggy.stolt@vbcf.ac.at
http://www.vbcf.ac.at/protech
The Vienna Biocenter Core Facilities (VBCF) is a research institute providing access to state of the art infrastructure and advanced research services on a fee-for-service basis. Additionally, VBCF core facilities are open to all forms of scientific cooperation. Information on general conditions of cooperation and usage fees is provided upon request.