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Isothermal Titration Calorimetry (ITC) is a method used to analyze biomolecular interactions by measuring the heat change that occurs when two molecules interact. Heat is liberated or absorbed as a result of the redistribution of non-covalent bonds when the interacting molecules go from the free to the bound state. The resulting isotherm is fitted to a binding model to obtain the affinity (KD), stoichiometry (N) and enthalpy of interaction (ΔH). The MicroCal PEAQ-ITC isothermal titration calorimeter from Malvern Instruments has a 200 μL sample cell and provides direct measurement of the heat absorbed or evolved as a result of mixing precise amounts of reactants in liquid samples.
-biomolecular interaction measurements as full service
Methoden & Expertise zur Forschungsinfrastruktur
The ProTech facility can run ITC analyses on the PEAQ-ITC instrument as a full service, or provide user training so that users can book the instrument and run their own experiments. Please contact the facility for more details.