Kurzbeschreibung
The NanoTemper Prometheus is a multi-purpose, label-free instrument measuring both intrinsic protein fluorescence and scattering while applying a defined temperature profile. Structural changes (folding/unfolding) as well as aggregation can be monitored over time, as a function of temperature, or under different chemical conditions. Since a sensitive fluorescence detection setup is used, only a minute protein sample is needed (>5µg/ml, 10µl). The Prometheus serves not only as a quality control instrument but also as a tool to find optimum conditions by using buffer screens to optimize stability. Using the Prometheus as a tool for day-to-day protein quality checks will improve the reproducibility of subsequent experiments that use purified proteins for structural and interaction studies while screening for potential binders can open up subsequent avenues of investigation.
https://nanotempertech.com/prometheus/
Ansprechperson
David Drechsel
Research Services
Please refer to: https://www.viennabiocenter.org/vbcf/protein-technologies/
Methoden & Expertise zur Forschungsinfrastruktur
Please refer to: https://www.viennabiocenter.org/vbcf/protein-technologies/