AVANCE III HD 600 MHz Spektrometer

University of Salzburg

Salzburg | Website

Large equipment

Short Description

Nuclear resonance spectrometer for applications of chemical structure elucidation, including small molecules and modern biological applications such as protein structure elucidation, protein interactions, of the analysis of post-translational modifications. The system consists of a superconducting magnet (14 Tesla), 1H/13C/15N/31P quadrupole probe head, sample changer for 24 samples, console (frequency generation, amplifier, lock, gradient unit), Temperature unit (-15 to 60 ° C), Linux Workstation, Ultra stabilized power supply (UPS for 11 min). The system can automatically measure sequentially up to 24 samples (typical for the elucidation of the chemical structure of small molecules) or highly complex experiments for the protein signal assignment and protein structure elucidation, extending over several days or weeks.

Contact Person

Prof. Dr. Chiara Cabrele

Research Services

Preliminary experiments for biomolecules (e.g., "fingerprint" 1H-15N two-dimensional protein spectrum) to determine whether a protein is suitable for NMR binding studies and / or three-dimensional NMR structure determination.

Methods & Expertise for Research Infrastructure

The device is used for the investigation of biological systems such as proteins, peptides and oligosaccharides. The applications range from the structural confirmation of peptides (chemical structure) to the three-dimensional structure determination of proteins.
A focus is the mapping of protein interaction sites with binding partners to the protein sequence as well as the localization of the binding on the ligand side.
Another focus is on the detection of post-translational modifications.
Smaller tasks are the chemical structure elucidation of small molecules, e.g. purified unknown substances, or the confirmation of the identity of substances which were acquired either commercially or from co-operation partners.
Furthermore, the chemically working groups at the University of Salzburg (for example, Prof. Nicola Hüsing, Department of Chemistry and Physics of Materials) are supported by routine measurements (one-dimensional 1H and 13C spectra).

Allocation to Core Facility

Chemical and Structural Biology

Prof. Dr. Chiara Cabrele
Fachbereich Biowissenschaften, Schwerpunkt ACBN
0043 662 8044 7240
chiara.cabrele@sbg.ac.at
https://www.uni-salzburg.at/index.php?id=208727
Please contact us via science.plus@sbg.ac.at, or contact the responsible person for this section, mentioned in the contact field
Department of Biosciences, University of Salzburg
Department of Chemistry and Physics of Materials, University of Salzburg
Wroclaw University of Science and Technology, Poland
Institute of Clinical Biochemistry, Hannover Medical School, Hannover, Germany
Department of Chemistry and Pharmacy, University of Regensburg, Germany
Institute of Microbiology, ETH Zurich, Switzerland
International PhD Program "Immunity in Cancer and Allergy"
01.08.2008-31.12.2017
Thalhamer Josef (Projektleiter), Aberger Fritz, Brandstetter Johann, Duschl Albert, Ferreira Fatima, Gratz Iris, Greil Richard, Hartmann Tanja, Huber Christian, Risch Angela, Wessler Silja
FWF

Kofaktor- und Substratabhängige Aktivierung des Gerinnungsfaktors IXa
01.07.2011-30.06.2014
Brandstetter Johann
FWF

Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
01.10.2013-30.09.2018
Huber Christian (Sprecher), Brandstetter Johann, Cabrele Chiara, Gadermaier Gabriele, Stutz Hanno
CD Forschungsgesellschaft, Sandoz, Thermo Fisher Scientific, BMWFJ
Novel Floral Scent Compounds from Night-Blooming Araceae Pollinated by Cyclocephaline Scarabs (Melolonthidae, Cyclocephalini)
2018
Maia ACD, Grimm C, Schubert M, Etl F, Gonçalves EG, Do Amaral Ferraz Navarro DM, Schulz S, Dötterl S
J. Chem. Ecol.
https://link.springer.com/article/10.1007%2Fs10886-018-1018-1
DOI: 10.1007/s10886-018-1018-1

A New Family of Capsule Polymerases Generates Teichoic Acid-Like Capsule Polymers in Gram-Negative Pathogens
2018
Litschko C, Oldrini D, Budde I, Berger M, Meens J, Gerardy-Schahn R, Berti F, Schubert M, Fiebig T
Mbio 9(3):E00641-18
https://mbio.asm.org/content/9/3/e00641-18/article-info
DOI: 10.1128/mBio.00641-18

The Recombinant Inhibitor of DNA Binding Id2 Forms Multimeric Structures via the Helix-Loop-Helix Domain and the Nuclear Export Signal
2018
Roschger C, Schubert M, Regl C, Andosch A, Marquez A, Berger T, Huber CG, Lütz-Meindl U, Cabrele C
Int. J. Mol. Sci. 19(4):E1105
https://www.mdpi.com/1422-0067/19/4/1105/htm
DOI: 10.3390/ijms19041105

Complete NMR Assignment of Succinimide and Its Detection and Quantification in Peptides and Intact Proteins
2017
Grassi L, Regl C, Wildner S, Gadermaier G, Huber CG, Cabrele C, Schubert M
Anal. Chem. 89(22):11962
https://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b01645
DOI: 10.1021/acs.analchem.7b01645

Two Distinct Conformations in Bet v 2 Determine Its Proteolytic Resistance to Cathepsin S
2017
Soh WT, Briza P, Dall E, Asam C, Schubert M, Huber S, Aglas L, Bohle B, Ferreira F, Brandstetter H
Int. J. Mol. Sci. 18(10):E2156
https://www.mdpi.com/1422-0067/18/10/2156
DOI: 10.3390/ijms18102156

Targeting of a Helix-Loop-Helix Transcriptional Regulator by a Short Helical Peptide
2017
Roschger C, Neukirchen S, Elsässer B, Schubert M, Maeding N, Verwanger T, Krammer B, Cabrele C
ChemMedChem 12(18):1497
https://onlinelibrary.wiley.com/doi/full/10.1002/cmdc.201700305
DOI: 10.1002/cmdc.201700305

A Secondary Structural Element in a Wide Range of Fucosylated Glycoepitopes
2017
Aeschbacher T, Zierke M, Smieško M, Collot M, Mallet JM, Ernst B, Allain FH, Schubert M
Chemistry 23(48):11598
https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201701866
DOI: 10.1002/chem.201701866

Impact of the amino acid sequence on the conformation of side chain lactam-bridged octapeptides
2017
Neukirchen S., Krieger V., Roschger C., Schubert M., Elsässer B., Cabrele C.
J. Pept. Sci. 23(7-8):587-596
https://onlinelibrary.wiley.com/doi/full/10.1002/psc.2997
DOI: 10.1002/psc.2997

Inhibition of delta-secretase improves cognitive functions in mouse models of Alzheimer's disease
2017
Z. Zhang, O. Obianyo, E. Dall, Y. Du, H. Fu, X. Liu, S.S. Kang, M. Song, S.P. Yu, C. Cabrele, M. Schubert, X. Li, J.Z. Wang, H. Brandstetter, K. Ye
Nat. Commun. 8:14740
https://www.nature.com/articles/ncomms14740
DOI: 10.1038/ncomms14740

Dimerization of the fungal defense lectin CCL2 is essential for its toxicity against nematodes
2017
S. Bleuler-Martinez, K. Stutz, R. Sieber, M. Collot, J.M. Mallet, M. Hengartner, M. Schubert, A. Varrot, M. Künzler
Glycobiology 27(5):486-500
https://academic.oup.com/glycob/article/27/5/486/2566822
DOI: 10.1093/glycob/cww113

Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8
2016
J. M. Propster, F. Yan, B. Ernst, F.H.-T. Allain, M. Schubert
Proc. Natl. Acad. Sci. USA 113, E4170-9
http://www.pnas.org/content/113/29/E4170.abstract
DOI: 10.1073/pnas.1602214113

Straightforward Solvothermal Synthesis toward Phase Pure Li2CoPO4F
2016
J. Schoiber, R.J.F. Berger , J. Bernardi, M. Schubert, C. Yada, H. Miki, N. Husing
Crystal Growth & Design 16, 4999-5005
Crystal Growth & Design 16, 4999-5005
DOI: 10.1021/acs.cgd.6b00573