Short Description
The Core Facility Flow Cytometry comprises the instrumentation for the investigation of immunological mechanisms at molecular and cellular level using the flow cytometers FACSCanto II (two devices), CytoFLEX S and Northern Lights 1000, the cell sorters FACSAria III and CytoFLEX SRT, a multiplex analyzer Magpix for simultaneous measurement of up to 50 analytes and an impedance flow cytometer Ampha Z32.
Contact Person
Univ.-Prof. Dr. Iris Gratz
Research Services
Flow cytometry, Luminex Analysis
Methods & Expertise for Research Infrastructure
Investigations of mechanisms of immune polarization (e.g. allergic immune reactions)
Signal transduction and gene expression in the immune system, the role of antigen-presenting cells in the skin, and the formation and recruitment of regulatory T cells
Development of vaccines based on recombinant proteins as well as DNA and RNA vaccines
Investigation of the immunotoxicity of nanomaterials.
The method park includes the common techniques of cellular and molecular immunology. Special expertise is available for complex mouse models with regard to allergic sensitization and allergic effector reactions as well as for immune reactions / immune regulation via the skin.
Equipment
Please contact the University of Salzburg (science_plus@plus.ac.at) or the responsible contact person for this section mentioned in the contact field.
Department of Ecology and Evolution, University of Salzburg
Department of Cell Biology, University of Salzburg
Department of Chemistry and Physics of Materials, University of Salzburg
Medical University of Vienna
Max Planck Institute, Martinsried, Germany
University of Innsbruck
University of Cologne, Germany
University of Erlangen/Nuremberg
University of Regensburg, Germany
DKFZ Heidelberg, Germany
Paul-Ehrlich-Institut, Langen, Germany
SIAF, Davos, Switzerland
Medical Research Council, Cambridge, UK
Weatherall Institute of Molecular Medicine, Oxford, UK
McMaster University, Hamilton, Canada
Stanford School of Medicine, USA
La Jolla Institute for Allergy and Immunology, USA
University of California, San Francisco, USA
NCI/NIH, USA
Academic Medical Center, Amsterdam, The Netherlands
Federal University of Rio de Janeiro, Brazil
Department of Cell Biology, University of Salzburg
Department of Chemistry and Physics of Materials, University of Salzburg
Medical University of Vienna
Max Planck Institute, Martinsried, Germany
University of Innsbruck
University of Cologne, Germany
University of Erlangen/Nuremberg
University of Regensburg, Germany
DKFZ Heidelberg, Germany
Paul-Ehrlich-Institut, Langen, Germany
SIAF, Davos, Switzerland
Medical Research Council, Cambridge, UK
Weatherall Institute of Molecular Medicine, Oxford, UK
McMaster University, Hamilton, Canada
Stanford School of Medicine, USA
La Jolla Institute for Allergy and Immunology, USA
University of California, San Francisco, USA
NCI/NIH, USA
Academic Medical Center, Amsterdam, The Netherlands
Federal University of Rio de Janeiro, Brazil
Immunity in cancer and allergy
2008-2017
Josef Thalhamer
FWF Doktoratskolleg W1213
Influence of fold-stability on allergenicity and immunogenicity
2014-2017
Richard Weiss
FWF P26997-B13
P.L.E.A.S.E. Vaccinate
2015
Richard Weiss
Pantec Biosolutions, Liechtenstein
Immune functions of epidermal Langerhans cells in vivo
2013-2016
Angelika Stöcklinger
FWF P25243-B22
Signaling crosstalk between NOD2 and IL-31
2013-2016
Jutta Horejs-Höck
FWF P25696
IL-31 regulates DC-mediated T cell responses in the course of atopic dermatitis
2011-2015
Jutta Horejs-Höck
FWF P23933
Immune regulation in the skin and induction of immunological tolerance
2014-2017
Iris Gratz
Debra Austria
Activation and maintenance of effector and regulatory T cells in the skin
2013-2016
Iris Gratz
R03AR064554 National Institute of Health (NIH), USA
The interplay between tree pollen allergens and their sources
2015-2018
Fatima Ferreira
FWF P27589
An innovative causal therapy for allergy: safe and rapid induction of an anti-inflammatory immune response using a mutant hypoallergen and vitamin D3
2014-2019
Fatima Ferreira
EU-FP7-Health-2013-Innovation-1 GA 601763
Mapping antibody profiles of Bet v 1 and associated food allergens during SIT
2014-2016
Michael Wallner
FWF P26125-B13
Immunomodulatory Effects of Ligand-binding to Pollen Allergens
2011-2016
Michael Wallner
FWF P23417-B13
2008-2017
Josef Thalhamer
FWF Doktoratskolleg W1213
Influence of fold-stability on allergenicity and immunogenicity
2014-2017
Richard Weiss
FWF P26997-B13
P.L.E.A.S.E. Vaccinate
2015
Richard Weiss
Pantec Biosolutions, Liechtenstein
Immune functions of epidermal Langerhans cells in vivo
2013-2016
Angelika Stöcklinger
FWF P25243-B22
Signaling crosstalk between NOD2 and IL-31
2013-2016
Jutta Horejs-Höck
FWF P25696
IL-31 regulates DC-mediated T cell responses in the course of atopic dermatitis
2011-2015
Jutta Horejs-Höck
FWF P23933
Immune regulation in the skin and induction of immunological tolerance
2014-2017
Iris Gratz
Debra Austria
Activation and maintenance of effector and regulatory T cells in the skin
2013-2016
Iris Gratz
R03AR064554 National Institute of Health (NIH), USA
The interplay between tree pollen allergens and their sources
2015-2018
Fatima Ferreira
FWF P27589
An innovative causal therapy for allergy: safe and rapid induction of an anti-inflammatory immune response using a mutant hypoallergen and vitamin D3
2014-2019
Fatima Ferreira
EU-FP7-Health-2013-Innovation-1 GA 601763
Mapping antibody profiles of Bet v 1 and associated food allergens during SIT
2014-2016
Michael Wallner
FWF P26125-B13
Immunomodulatory Effects of Ligand-binding to Pollen Allergens
2011-2016
Michael Wallner
FWF P23417-B13
Fold stability during endolysosomal acidification is a key factor for allergenicity and immunogenicity of the major birch pollen allergen
2016
Machado Y., Freier R., Scheiblhofer S., Thalhamer T., Mayr M., Briza P., Grutsch S., Ahammer L., Fuchs J.E., Wallnoefer H.G., Isakovic A., Kohlbauer V., Hinterholzer A., Steiner M., Danzer M., Horejs-Hoeck J., Ferreira F., Liedl K.R., Tollinger M., Lackner P., Johnson C.M., Brandstetter H., Thalhamer J., Weiss R.
J Allergy Clin Immunol. 2015 Nov 11. pii: S0091-6749(15)01366-4.
http://dx.doi.org/10.1016/j.jaci.2015.09.026
Protease recognition sites in Bet v 1a are cryptic, explaining its slow processing relevant to its allergenicity
2015
Freier R., Dall E., Brandstetter H.
Sci Rep. 5:12707
http://dx.doi.org/10.1038/srep12707
HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis
2015
Wolkerstorfer S., Schwaiger E., Rinnerthaler M., Karina Gratz I., Zoegg T., Brandstetter H., Achatz-Straussberger G.
Cell Mol Immunol.
http://dx.doi.org/10.1038/cmi.2015.018
Nanoparticle–allergen interactions mediate human allergic responses: protein corona characterization and cellular responses.
2016
Radauer-Preiml I., Andosch A., Hawranek T., Luetz-Meindl U., Wiederstein M., Horejs-Hoeck J., Himly M., Boyles M.S.P., Duschl A.
Particle and Fibre Toxicology. 13:3
DOI: 10.1186/s12989-016-0113-0
Antigenic determinants of the bilobal cockroach allergen Bla g 2
2015
Woodfolk, J.A., Glesner J., Wright P.W., Kepley C.L., Li M., Himly M., Muehling L.M., Gustchina A., Wlodawer A., Chapman M., Pomes A.
J Biol Chem. 291(5): 2288-301. Epub 2015 Dec 7.
DOI: 10.1074/jbc.M115.702324
Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure under different cell culture conditions
2015
Stoehr L.C., Endes C., Radauer-Preiml I., Boyles M.S.P., Casals E., Balog S., Pesch M., Petri-Fink A., Rothen-Rutishauser B., Himly M., Clift M.J.D., Duschl A.
Particle and Fibre Toxicology. 12:29
DOI: 10.1186/s12989-015-0104-6
Prerequisites for functional interleukin 31 signaling and its feedback regulation by suppressor of cytokine signaling 3 (SOCS3)
2015
Maier E., Mittermeir M., Ess S., Neuper T., Schmiedlechner A., Duschl A., Horejs-Hoeck J.
J Biol. Chem. 290, 24747-24759
DOI: 10.1074/jbc.M115.661306
Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice
2015
Diesner S.C., Schultz C., Ackaert C., Oostingh G.J., Ondracek A., Stremnitzer C., Singer J., Heiden D., Roth-Walter F., Fazekas J., Assmann V.E., Jensen-Jarolim E., Stutz H., Duschl A., Untersmayr E.
PLoS One. 2015 May 8;10(5): e0126279
DOI:10.1371/journal.pone.0126279. eCollection 2015.
Wave of Regulatory T Cells into Neonatal Skin Mediates Tolerance to Commensal Microbes
2015
Scharschmidt T.C., Vasquez K.S., Truong H.A., Gearty S.V., Pauli M.L., Nosbaum A., Gratz I.K., Otto M., Moon J.J., Liese J., Abbas A.K., Fischbach M.A., Rosenblum MD.A.
Immunity
DOI: 10.1016/j.immuni.2015.10.016.
UV irradiation-induced inflammation, what is the trigger?
2015
Gratz I.K., Kofler B.
Experimental Dermatology Volume: 24 Issue: 12 Pages: 916-917
DOI: 10.1111/exd.12849
Immune repertoire profiling reveals that clonally expanded B and T cells infiltrating diseased human kidneys can also be tracked in blood
2015
Weinberger J., Jimenez-Heredia R., Schaller S., Suessner S., Sunzenauer J., Reindl-Schwaighofer R., Weiss R., Winkler S., Gabriel C., Danzer M., Oberbauer R.
PlosOne 10(11)
Prophylactic mRNA vaccination against allergy confers long-term memory responses and persistent protection in mice
2015
Hattinger E., Scheiblhofer S., Roesler E., Thalhamer T., Thalhamer J. and Weiss R.
J Immunol Res
DOI: 10.1155/2015/797421.
What Is the Antiallergic Potential of DNA Vaccination?
2015
Weiss R., S. Scheiblhofer and J. Thalhamer
Immunotherapy 22:1-4
Immune Reactions against Gene Gun Vaccines Are Differentially Modulated by Distinct Dendritic Cell Subsets in the Skin
2015
Weber C.S., Hainz K., Deressa T., Strandt H., Florindo Pinheiro D., Mittermair R., Pizarro Pesado J., Thalhamer J., Hammerl P, Stoecklinger A.
PLoS One 1;10(6)
Natural clinical tolerance to peanut in African patients is caused by poor allergenic activity of peanut IgE
2015
Wollmann E., Hamsten C., Sibanda E., Ochome M., Focke-Tejkl M., Asarnoj A., Önell A., Lilja G., Gallerano D., Lupinek C., Thalhamer T., Weiss R., Thalhamer J., Wickman M., Valenta R., van Hage M.
Allergy. 70(6): 638-52
J. Pollen-derived non-allergenic substances enhance Th2-induced IgE production in B cells
2015 (epub ahead of print).
Oeder S., Alessandrini F., Wirz O.F., Braun A., Wimmer M., Frank U., Hauser M., Durner J., Ferreira F., Ernst D., Mempel M., Gilles S., Buters J.T.M., Behrendt H., Traidl-Hoffmann C., Schmidt-Weber C., Akdis M., Gutermuth J.
Allergy
DOI: 10.1111/all.12707
Pectate lyase allergens: sensitization profiles and cross-reactivity pattern
2015
Pichler U., Hauser M., Wolf M., Bernardi M.L., Gadermaier G., Weis, R., Ebner C., Yokoi H., Takai T., Didierlaurent D., Rafaiani C., Briza P., Mari A., Behrendt H., Wallner M., Ferreira F.
Plos One 10(5): e0120038
DOI: 10.1371/journal.pone.0120038.
Correlation of sensitization capacity and T-cell recognition within the Bet v 1 family. J. Allergy Clin
2015
Kitzmüller C., Zulehner N., Roulias A., Briza P., Ferreira F., Faé I., Fischer G.F., Bohle B.
Immunol. 136: 151-158
DOI: 10.1016/j.jaci.2014.12.1928
Tree pollen allergens-an update from a molecular perspective
2015
Asam C., Hofer H., Wolf M., Aglas L., Wallner M.
Allergy.
DOI: 10.1111/all.12696.
2016
Machado Y., Freier R., Scheiblhofer S., Thalhamer T., Mayr M., Briza P., Grutsch S., Ahammer L., Fuchs J.E., Wallnoefer H.G., Isakovic A., Kohlbauer V., Hinterholzer A., Steiner M., Danzer M., Horejs-Hoeck J., Ferreira F., Liedl K.R., Tollinger M., Lackner P., Johnson C.M., Brandstetter H., Thalhamer J., Weiss R.
J Allergy Clin Immunol. 2015 Nov 11. pii: S0091-6749(15)01366-4.
http://dx.doi.org/10.1016/j.jaci.2015.09.026
Protease recognition sites in Bet v 1a are cryptic, explaining its slow processing relevant to its allergenicity
2015
Freier R., Dall E., Brandstetter H.
Sci Rep. 5:12707
http://dx.doi.org/10.1038/srep12707
HAX1 deletion impairs BCR internalization and leads to delayed BCR-mediated apoptosis
2015
Wolkerstorfer S., Schwaiger E., Rinnerthaler M., Karina Gratz I., Zoegg T., Brandstetter H., Achatz-Straussberger G.
Cell Mol Immunol.
http://dx.doi.org/10.1038/cmi.2015.018
Nanoparticle–allergen interactions mediate human allergic responses: protein corona characterization and cellular responses.
2016
Radauer-Preiml I., Andosch A., Hawranek T., Luetz-Meindl U., Wiederstein M., Horejs-Hoeck J., Himly M., Boyles M.S.P., Duschl A.
Particle and Fibre Toxicology. 13:3
DOI: 10.1186/s12989-016-0113-0
Antigenic determinants of the bilobal cockroach allergen Bla g 2
2015
Woodfolk, J.A., Glesner J., Wright P.W., Kepley C.L., Li M., Himly M., Muehling L.M., Gustchina A., Wlodawer A., Chapman M., Pomes A.
J Biol Chem. 291(5): 2288-301. Epub 2015 Dec 7.
DOI: 10.1074/jbc.M115.702324
Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure under different cell culture conditions
2015
Stoehr L.C., Endes C., Radauer-Preiml I., Boyles M.S.P., Casals E., Balog S., Pesch M., Petri-Fink A., Rothen-Rutishauser B., Himly M., Clift M.J.D., Duschl A.
Particle and Fibre Toxicology. 12:29
DOI: 10.1186/s12989-015-0104-6
Prerequisites for functional interleukin 31 signaling and its feedback regulation by suppressor of cytokine signaling 3 (SOCS3)
2015
Maier E., Mittermeir M., Ess S., Neuper T., Schmiedlechner A., Duschl A., Horejs-Hoeck J.
J Biol. Chem. 290, 24747-24759
DOI: 10.1074/jbc.M115.661306
Nitration of β-Lactoglobulin but Not of Ovomucoid Enhances Anaphylactic Responses in Food Allergic Mice
2015
Diesner S.C., Schultz C., Ackaert C., Oostingh G.J., Ondracek A., Stremnitzer C., Singer J., Heiden D., Roth-Walter F., Fazekas J., Assmann V.E., Jensen-Jarolim E., Stutz H., Duschl A., Untersmayr E.
PLoS One. 2015 May 8;10(5): e0126279
DOI:10.1371/journal.pone.0126279. eCollection 2015.
Wave of Regulatory T Cells into Neonatal Skin Mediates Tolerance to Commensal Microbes
2015
Scharschmidt T.C., Vasquez K.S., Truong H.A., Gearty S.V., Pauli M.L., Nosbaum A., Gratz I.K., Otto M., Moon J.J., Liese J., Abbas A.K., Fischbach M.A., Rosenblum MD.A.
Immunity
DOI: 10.1016/j.immuni.2015.10.016.
UV irradiation-induced inflammation, what is the trigger?
2015
Gratz I.K., Kofler B.
Experimental Dermatology Volume: 24 Issue: 12 Pages: 916-917
DOI: 10.1111/exd.12849
Immune repertoire profiling reveals that clonally expanded B and T cells infiltrating diseased human kidneys can also be tracked in blood
2015
Weinberger J., Jimenez-Heredia R., Schaller S., Suessner S., Sunzenauer J., Reindl-Schwaighofer R., Weiss R., Winkler S., Gabriel C., Danzer M., Oberbauer R.
PlosOne 10(11)
Prophylactic mRNA vaccination against allergy confers long-term memory responses and persistent protection in mice
2015
Hattinger E., Scheiblhofer S., Roesler E., Thalhamer T., Thalhamer J. and Weiss R.
J Immunol Res
DOI: 10.1155/2015/797421.
What Is the Antiallergic Potential of DNA Vaccination?
2015
Weiss R., S. Scheiblhofer and J. Thalhamer
Immunotherapy 22:1-4
Immune Reactions against Gene Gun Vaccines Are Differentially Modulated by Distinct Dendritic Cell Subsets in the Skin
2015
Weber C.S., Hainz K., Deressa T., Strandt H., Florindo Pinheiro D., Mittermair R., Pizarro Pesado J., Thalhamer J., Hammerl P, Stoecklinger A.
PLoS One 1;10(6)
Natural clinical tolerance to peanut in African patients is caused by poor allergenic activity of peanut IgE
2015
Wollmann E., Hamsten C., Sibanda E., Ochome M., Focke-Tejkl M., Asarnoj A., Önell A., Lilja G., Gallerano D., Lupinek C., Thalhamer T., Weiss R., Thalhamer J., Wickman M., Valenta R., van Hage M.
Allergy. 70(6): 638-52
J. Pollen-derived non-allergenic substances enhance Th2-induced IgE production in B cells
2015 (epub ahead of print).
Oeder S., Alessandrini F., Wirz O.F., Braun A., Wimmer M., Frank U., Hauser M., Durner J., Ferreira F., Ernst D., Mempel M., Gilles S., Buters J.T.M., Behrendt H., Traidl-Hoffmann C., Schmidt-Weber C., Akdis M., Gutermuth J.
Allergy
DOI: 10.1111/all.12707
Pectate lyase allergens: sensitization profiles and cross-reactivity pattern
2015
Pichler U., Hauser M., Wolf M., Bernardi M.L., Gadermaier G., Weis, R., Ebner C., Yokoi H., Takai T., Didierlaurent D., Rafaiani C., Briza P., Mari A., Behrendt H., Wallner M., Ferreira F.
Plos One 10(5): e0120038
DOI: 10.1371/journal.pone.0120038.
Correlation of sensitization capacity and T-cell recognition within the Bet v 1 family. J. Allergy Clin
2015
Kitzmüller C., Zulehner N., Roulias A., Briza P., Ferreira F., Faé I., Fischer G.F., Bohle B.
Immunol. 136: 151-158
DOI: 10.1016/j.jaci.2014.12.1928
Tree pollen allergens-an update from a molecular perspective
2015
Asam C., Hofer H., Wolf M., Aglas L., Wallner M.
Allergy.
DOI: 10.1111/all.12696.