Short Description
The Axio Imager M2 is an upright widefield microscope with the possibility for transmission light and fluorescence. The system is characterized by a powerful image contrast, perfect homogeneity and a very good resolution. This microscope is equiped with a CCD camera and connected to a workstation.
The system supports following applications (- or combinations thereof):
Transmission- and multifluorescence acquisition of fixed samples
Non – confocal applications
Polarized Light Microscopy
Technical Data:
Wide Field Illumination
Halogen lamp 12V , 100W
HBO 100
Camera
MRC5: CCD Camera
5 Megapixel resolution
Interlaced and progressive readout
12 different capture modes
Binning from 1x1 to 10x10 for increased sensitivity
Dynamic range 1:1300
Fast live image with up to 14 frames/second
Large field of view and low noise due to 2/3‘‘ CCD
Trigger In/Out signal for control of external components
Complete imaging software with measuring functions
Filter Sets
DAPI
GFP
Cy3
Cy5
Objectives
5x/ 0.16 Plan-Apochromat
10x/ 0.45 Plan-Apochromat
20x/ 0.8 Plan-Apochromat
40x/ 1.3 Plan-Neofluar, Oil
100x/ 1.4 Plan-Apochromat, Oil, DIC
Software
ZEN blue 2012 + Analyse
Furthermore
Motorized Z stage
on request Dip In objectives (W N-Achroplan 20x/0.5; W N-Achroplan 63x/0.9)
Fees
18€ / h
Contact Person
Sabine Rauscher
Research Services
Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance
Methods & Expertise for Research Infrastructure
The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies.
The available systems support high resolution imaging of tissue sections or cells, 3D and 4D acquisition, visualization of protein-protein interactions (like FRET) and live cell imaging. Furthermore the facility offers workstations to the users, which allow an onsite evaluation and processing of their data with the suitable software.
In addition the area of microscopy the core facility houses a small histology unit for sample preparation. This is equipped with one cryostat and two microtomes. The microtomes are only to cut paraffin embedded material for the purpose of RNA and DNA extraction.
For performing live cell imaging the facility provides a fully equipped cell culture lab.
To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
Allocation to research infrastructure
The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!
fee (AxioImager M2): 18 €/h