Axiovert cell culture microscope

Medical University of Vienna

Wien | Website

Large equipment

Short Description

The Axiovert is an inverted microscope supporting transmission light and fluorescence. On this microscope cells in plates and dishes can be photographed. It is equipped with a CCD camera and connected to a workstation.

The system supports following applications (- or combinations thereof) by Transmission light- and multifluorescence imaging of:
Cell cultures
Time Series

Technical Data:

Wide Field Illumination
Halogen lamp 12V , 100W
HBO 50

Camera
AxioCam ICc3: CCD Camera
5 Megapixel Resolution
fast live Imaging up to 15 Frames/Second
1920 x 1080 Pixels

Filter Sets
DAPI
GFP
Rhodamin

Objectives
5x/ 0.12 A-Plan
10x/ 0.25 A-Plan
20x/ 0.3 LD A-Plan Ph1
40x/ 0.5 LD A-Plan Ph2


Software
ZEN blue 2012

Furthermore
Time Series
Bioflux – Flow Chamber
Ibidi - Flow Chambers

Fees
8€
18€/h Microscope + Flow Chambers

Contact Person

Marion Gröger, Sabine Rauscher

Research Services

Advice and support in setting up protocols
Trainings for the equipment of the facility
Assistance in using the equipment
Data analysis
Imaging Workshops
Equipment maintenance

Methods & Expertise for Research Infrastructure

The Core Facility Imaging is located in the Anna Spiegel Building – Center of Translational Research. In the area of Imaging a large variety of techniques is available to address complex problems in vitro, in situ and in vivo. The Imaging Core Facility provides state-of-the-art equipment for committed work in this field. Upon request, experienced staff members offer their support and know-how for establishing and troubleshooting of novel experimental strategies.

The available systems support high resolution imaging of tissue sections or cells, 3D and 4D acquisition, visualization of protein-protein interactions (like FRET) and live cell imaging. Furthermore the facility offers workstations to the users, which allow an onsite evaluation and processing of their data with the suitable software.

In addition the area of microscopy the core facility houses a small histology unit for sample preparation. This is equipped with one cryostat and two microtomes. The microtomes are only to cut paraffin embedded material for the purpose of RNA and DNA extraction.

For performing live cell imaging the facility provides a fully equipped cell culture lab.

To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.

The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!

Allocation to Core Facility

Core Facility Imaging

To use the instruments in the facility a training given by the facility staff is an indispensable prerequisite. It is prohibited to use microscopes without this training and moreover users who have been trained are not permitted to train other users! During the training users will get the login data for our booking calendar and are then able to book the systems by themselves.

The opening hours of the facility are Mo.-Fr. between 9.00 and 17.00. It is possible to use the facility out of opening hours, however in this case please contact the facility staff early enough in order to ensure practical arrangements!

fee (Axiovert cell culture microscope): 8 €