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The Dual HPLC system enables simultaneous or parallel separation of samples on two separation columns. These columns can be combined into a multidimensional separation system or they can be used to speed up the sample analysis. A combination of different column dimensions and chemistry is possible.
Multidimensional separation of peptides. Online digest of proteins by using immobilized enzymes. Dual separation approach for fast sample throughput.
Methods & Expertise for Research Infrastructure
A series of chromatographic methods has been developed for the use with the dual gradient system. The Proteomics Core Facility uses the Dual Gradient system for on-line digestion of biological samples and for the online multidimensional separation.