Forschungsinfrastruktur

Vienna Biocenter Core Facilities GmbH (VBCF)

Wien | Website

Short Description

Fluorescence Lifetime Imaging Microscope (FLIM) based on the MicroTime 200 by PicoQuant.
For more details see: http://www.vbcf.ac.at/facilities/advanced-microscopy/?o=157

Contact Person

Kareem Elsayad

Research Services

The VBCF Advanced Microscopy Facility provides access, training and technical support for researchers to an expanding inventory of cutting edge optical imaging techniques.

Fluorescence Lifetime Imaging Microscope (FLIM) based on the MicroTime 200 by PicoQuant.
For more details and current possibilities with this instrument see:
http://www.vbcf.ac.at/facilities/advanced-microscopy/?o=157

Methods & Expertise for Research Infrastructure

Time-resolved confocal scanning Fluorescence Lifetime Imaging Microscope (Microtime 200, Picoquant), suitable for:
1) Fluorescence Lifetime Imaging (time domain), e.g. life-time FRET
2a) Fluorescence Correlation & Cross-Correlation Spectroscopy (FCS & FCCS)
2b) Fluorescence Lifetime Correlation & Cross-Correlation Spectroscopy (FLCS & FLCCS)
2c) Other spatial/temporal fluorescence correlation studies
3) Steady-state and Time-resolved Fluorescence Anisotropy (TRFA) - polarization sensivitive lifetime measurements
4) Photon Antibunching measurements

Specs:
- two hybrid PMA detectors
- 4 laser lines (rep rate 0-40MHz): 440nm, 485nm, 510nm, 561nm. Possibility of pulse interlieved (PIE) excitation
- Piezo Objective-scanning.
- Temperature control for live cell imaging (for sample & objective lens)
- 355nm kHz-picosecond laser for inducing e.g. DNA damage at selected areas
- Analysis software: Symphotime 64 (Picoquant), Fluofit (Picoquant), custom written software

Allocation to Core Facility

Advanced Microscopy