Kristallisationsroboter mosquito Xtal3

University of Salzburg

Salzburg | Website

Large equipment

Short Description

The device consists of: 8-channel pipetting unit; Coil with 26,000 pipettes; Pipette loading unit; Calibration block; Guide block; Welding bracket for hanging drop applications; Computer; Control and evaluation software. The device allows reproducible pipetting steps in the volume range 25 nl to 1.2 µl with highly viscous liquids, a humidity chamber provides protection against evaporation of the nanoliter drops.

Contact Person

Prof. Dr. Johann Brandstetter

Research Services

crystallisation campaigns

Methods & Expertise for Research Infrastructure

The crystallisation of a biomolecule represents a major challenge in the determination of its three-dimensional structure. The crystallisation robot enables the miniaturization of crystallisation campaigns to the nanolitre scale. This reduction enables an up to 100-fold reduction of the required protein, or conversely, a corresponding depth increase of the crystallization campaigns with identical protein usage. Importantly, this nanoliter robotics allow to carry out structural investigations on proteins, which can be produced at inherently low yield, e.g., membrane proteins. Finally, by its unique contamination-free working procedure the robot also qualifies for sensitive pipetting applications such as (epi) genomics.

Allocation to Core Facility

Chemical and Structural Biology

Prof. Dr. Johann Brandstetter
Fachbereich Biowissenschaften, Schwerpunkt ACBN
+43 662 8044 7270
hans.brandstetter@sbg.ac.at
https://www.plus.ac.at/biowissenschaften/
Please contact the research service of the University of Salzburg (Technologie@sbg.ac.at) or the responsible contact person for this section mentioned in the contact field.
Department of Biosciences, University of Salzburg
SALK, PMU
Medizinische Universität Wien
Technische Universität München
Novo Nordisk Kopenhagen
Universität Köln
Helmholtz Forschungszentrum Jülich
Emory University, Medical School (USA)
MPI Martinsried
Universidade Federal de Minas Gerais, Brasilien
International PhD Program "Immunity in Cancer and Allergy"
01.08.2008 - 31.12.2017
Thalhamer Josef (Projektleiter); Aberger Fritz, Brandstetter Johann, Duschl Albert, Ferreira Fatima, Gratz Iris, Greil Richard, Hartmann Tanja, Huber Christian, Risch Angela, Wessler Silja
FWF
http://www.uni-salzburg.at/index.php?id=86&MP=200731-200747

Christian Doppler Laboratory for Innovative Tools for the Characterization of Biosimilars
01.10.2013-30.06.2021
Huber christian (Sprecher); Brandstetter Johann, Cabrele Chiara, Gadermaier Gabriele, Stutz Hanno
CD Forschungsgesellschaft, Sandoz, Thermo Fisher Scientific, bmWFJ
http://cdl-biosimilars.sbg.ac.at/

Entwicklung konformationsspezifischer Furin-Inhibitoren
13.05.2020-12.05.2022
Sven Dahms, Hans Brandstetter
FWF
https://fwf.ac.at
Phosphonate as Stable Zinc‐binding Group for Inhibitors of Clostridial Collagenase H (ColH) as Pathoblocker Agents
2021
Katrin Voos, Esther Schönauer, Alaa Alhayek, Jörg Haupenthal, Anastasia Andreas, Rolf Müller, Rolf W Hartmann, Hans Brandstetter, Anna KH Hirsch, Christian Ducho
ChemMedChem
https://doi.org/10.1002/cmdc.202000994
https://doi.org/10.1002/cmdc.202000994

The Basicity Makes the Difference: Improved Canavanine-Derived Inhibitors of the Proprotein Convertase Furin
2021
Thuy Van Lam van, Miriam Ruth Heindl, Christine Schlutt, Eva Böttcher-Friebertshäuser, Ralf Bartenschlager, Gerhard Klebe, Hans Brandstetter, Sven O Dahms, Torsten Steinmetzer
ACS Medicinal Chemistry Letters
https://doi.org/10.1021/acsmedchemlett.0c00651
https://doi.org/10.1021/acsmedchemlett.0c00651