Leica EM Cryo-CLEM

Institute of Science and Technology Austria (IST Austria)

Klosterneuburg | Website

Large equipment

Short Description

Leica EM Cryo CLEM set for widefield light microscopy for analysis of cryo samples in the cryo light and electron microscope.

- Microscope stand

- Camera
Leica DFC9000 GT: deep-cooled 4.2 MP sCMOS camera with a maximum of 82% quantum efficiency, 50 frames per second acquisition speed, and extreme low dark current of 0.14e/p/sec, pixel size: 6,5 μm, Bit depth: 12/16 bit.

- Objective lens
Leica HCX PL APO 50x / 0.90 CLEM objective. Working distance of 0.28 mm. Maximum resolution of 512 nm.

- Filter system
DAPI ET: Excitation: BP 350/50; Dichroic: LP 400, Emission: BP 460/50
GFP ET: Excitation: 470/40; Dichroic: 495; Emission: 525/50
TXR ET: Excitation: BP 560/40; Dichroic: LP 585; Emission: BP 630/75

- Software
CLEM Viewer

Contact Person

Ludek Lovicar

Research Services

Widefield light microscopy for analysis of cryo samples.

Methods & Expertise for Research Infrastructure

Cryo-Corellative Light and Electron Microscopy

Allocation to Core Facility

Electron Microscopy Facility

Information on terms of use, cooperation and fees is provided upon request. All such information is defined in scientific collaboration agreement.