Institute of Science and Technology Austria (ISTA)
Klosterneuburg | Website
Short Description
Cryo Electron Microscopes
- 300kV Cryo-TEM Titan Krios G3i – Single Particle Analysis and Cryo-Electron Tomography
- 200kV Cryo-TEM Glacios – Single Particle Analysis and Cryo-Electron Tomography
- Cryo-FIB/SEM Aquilos – Cryo-lamella preparation
- Leica EM Cryo-CLEM
- Cryo tomography transfer system Fischione M2550
Electron Microscopes
- Jeol JEM2800 / 200kV - Scanning/transmission electron microscope
- Tecnai 10 / 100kV - Transmission electron microscope
- Tecnai 12 / 120kV - Transmission electron microscope
- FE-SEM Merlin VP Compact / 3D array Tomography SEM
Equipment for sample preparation
- Leica EM UC7; ultramicrotome
- Leica EM FC7; cryochamber for Leica EM UC7
- Leica Ultracut UCT; ultramicrotome
- Leica EM KMR3; glass knife maker
- High pressure freezing BALTEC HPM010
- Freeze fracture machine Leica EM ACE900
- Freeze fracture machine Jeol JFD V
- High vacuum carbon coater and metal sputter
- Fully automated critical point dryer Leica CPD300
- Dual chamber glow discharge unit ELMO
- Automatic freeze-substitution apparatus / 2 x Leica EM AFS
- Plunge freezing apparatus / Leica EM CPC/ FEI Vitrobot Mark IV
- Automated immunogold labeling system / Leica EM IGL
- Automatic section stainer / Leica EM stain
- Hybri-oven / Hybridization incubator
- Sandbluster
Data analysis
- Powerful workstation with TEMography software (reconstruction and visualization) for tomography data processing, Fiji, Matlab, Stacker.
- HD touchscreen Wacom Cintiq 22HD.
- Powerful Workstation with off-line Atlas Array Tomography software for Atlas Array Tomography data processing.
- 10TB data storage unit for Atlas Array Tomography data.
- 2x 300TB storage with 4 GPU for On-The-Fly processing and post processing of cryo-EM data
Contact Person
Ludek Lovicar
Research Services
• Biological sample preparation for electron microscopy:
◦ Conventional room temperature fixation
◦ Cryo fixation (High pressure freezing, plunge freezing, freeze substitution, freeze fracturing)
◦ Immunolabelling
◦ Freeze-fracture replica electron microscopy combined with SDS digestion
◦ Correlative microscopy
◦ Ultramicrotomy
◦ Negative staining
◦ Chemical & critical point drying
◦ Metal sputtering
◦ Carbon coating
• Development of biological sample preparation techniques
• Lamella preparation by FIB (biology, chemistry)
• Imaging by means of electron microscopy:
◦ TEM (room temperature & cryo)
◦ STEM
◦ SEM
◦ S/TEM EDS analysis
◦ S/TEM in situ heating experiments
◦ 3D Tomography
◦ Array tomography (3D SEM)
◦ Cryo-TEM
◦ Cryo-FIB
• User training in biological sample preparation for electron microscopy and Cryo-Electron Microscopy, training in imaging by means of electron microscopy and Cryo-Electron Microscopy, and training in EM data post-processing.
• Image processing and data analysis: TEMography, Fiji, TrakEM, Matlab, Atlas Array Tomography, Stacker, Reconstructer.
Methods & Expertise for Research Infrastructure
High performance EM-equipment & sample preparation equipment & highly skilled crew.
Equipment
- Jeol JEM2800 scanning/transmission electron microscope (200kV)
- Transmission Electron Microscope FEI Tecnai 12
- Transmission Electron Microscope FEI Tecnai 10 (100kV) equipped with TEM side-mounted camera EMSIS MegaView III G3
- Field-Emission Scanning Electron Microscope Carl Zeiss Merlin Compact VP
- Plunge freezer FEI Vitrobot Mark IV
- High vacuum coating system Leica EM ACE600
- Automatic ultramicrotome - ATUMtome ATUM500
- Plunge freezer Leica EM CPC
- Cryo-transfer system Fischione M2550 + Turbo Pumping Station Model 9030
- Automated Critical Point Dryer Leica EM CPD300
- 300KV Cryo-TEM Titan Krios G3i
- 200kV Cryo-TEM Glacios
- Cryo-FIB/SEM Aquilos
- Leica EM Cryo-CLEM
- Plunge freezer Leica EM GP2
- Freeze Fracture System Leica EM ACE900